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Effects of Cryptococcus neoformans on activation of human alveolar basal epithelial adenocarcinoma cells (A549 cells).

Introduction: The bronchial epithelium has defense mechanisms based on innate immunity against various airborne microorganisms, including Cryptococcus neoformans. Purpose: We evaluated the inflammatory effects of C. neoformans using human alveolar basal epithelial adenocarcinoma cells (A549 cells)....

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Published in:Minas Gerais Medical Journal / Revista Médica de Minas Gerais Vol. 34; no. 4; pp. 1 - 8
Main Authors: da Cruz Freitas, Karina Cristina, Maria Santos, Wanessa, Mahler Pereira, Aline Beatriz, Euripedes Andrade-Silva, Leonardo, Roberto da Silva, Paulo, Silva Vergara, Mario Leon, de Paula Rogerio, Alexandre
Format: research tables/charts Journal Article
Published: Associacao Medica de Minas Gerais 2024
Online Access:View this record in EBSCOhost
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      dt: 2024
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      pub: Associacao Medica de Minas Gerais
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        10.5935/2238-3182.2024e34111-en
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        atl: Effects of Cryptococcus neoformans on activation of human alveolar basal epithelial adenocarcinoma cells (A549 cells).
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        au:
          da Cruz Freitas, Karina Cristina
          Maria Santos, Wanessa
          Mahler Pereira, Aline Beatriz
          Euripedes Andrade-Silva, Leonardo
          Roberto da Silva, Paulo
          Silva Vergara, Mario Leon
          de Paula Rogerio, Alexandre
        affil: Institute of Health Sciences, Department of Biomedicine, Laboratory of Experimental Immunopharmacology, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil
      sug:
        subj:
          Cryptococcosis Complications
          Pulmonary Alveoli Pathology
          Adenocarcinoma of Lung
          Neoplasms, Glandular and Epithelial
          Cell Line, Tumor Physiology
          Cell Line, Tumor Metabolism
          Meningoencephalitis Etiology
          Meningoencephalitis Microbiology
          Human
          Brazil
          In Vitro Studies
          Cytokines
          Interleukins
          Transforming Growth Factor beta
          Phosphorylation
          Transcription Factors Metabolism
          Phagocytosis
          Tumor Markers, Biological
          Cryptococcosis Immunology
          Funding Source
      ab:
        Introduction: The bronchial epithelium has defense mechanisms based on innate immunity against various airborne microorganisms, including Cryptococcus neoformans. Purpose: We evaluated the inflammatory effects of C. neoformans using human alveolar basal epithelial adenocarcinoma cells (A549 cells). Methods: A549 cells were exposed to C. neoformans (Multiplicity of Infection - MOI 1:100) and the analyses were performed 24 hours after stimulation. Results: C. neoformans increased the production of IL-6 and TGF-ß1, but not IL-8 and IL-10, and activated ERK1/2 and NF-B phosphorylation in A549 cells when compared to the control group. There was a greater adhesion of C. neoformans when compared to internalization in A549 cells. Conclusion: The C. neoformans is able to promote changes in inflammatory parameters in human adenocarcinoma alveolar basal epithelial cells.
        Introdução: O epitélio brônquico possui mecanismos de defesa baseados na imunidade inata contra diversos microrganismos veiculados pelo ar, entre eles o Cryptococcus neoformans. Objetivo: Avaliamos os efeitos inflamatórios de C. neoformans usando células de adenocarcinoma epitelial basal alveolar humano (células A549). Métodos: Células A549 foram expostas ao C. neoformans (Multiplicidade de Infecção - MOI 1:100) e as análises foram realizadas 24 horas após o estímulo. Resultados: C. neoformans aumentou a produção de IL-6 e TGF-ß1, mas não de IL-8 e IL-10, e ativou a fosforilação de ERK1/2 e NF-B em células A549 quando comparado ao grupo controle. Houve maior adesão de C. neoformans quando comparada à internalização em células A549. Conclusão: O C. neoformans é capaz de promover alterações nos parâmetros inflamatórios em células epiteliais basais alveolares de adenocarcinoma humano.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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