Design, fabrication, and characterization of a user-friendly microfluidic device for studying liver zonation-on-chip (ZoC)
Liver zonation is a fundamental characteristic of hepatocyte spatial heterogeneity, which is challenging to recapitulate in traditional cell cultures. This study presents a novel microfluidic device designed to induce zonation in liver cell cultures by establishing an oxygen gradient using standard...
| Publicado en: | Biomedical Microdevices Vol. 27; no. 1; pp. 1 - 17 |
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| Autores principales: | , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Mar2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=183067151&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 183067151 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Mar2025 vid: 27 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 183067151 10.1007/s10544-025-00738-1 183067151 ppf: 1 ppct: 16 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Design, fabrication, and characterization of a user-friendly microfluidic device for studying liver zonation-on-chip (ZoC) aug: au: Mahdavi, Reza Hashemi-Najafabadi, Sameereh Ghiass, Mohammad Adel Valaskivi, Silmu Välimäki, Hannu Kreutzer, Joose Hamngren Blomqvist, Charlotte Romeo, Stefano Kallio, Pasi Adiels, Caroline Beck affil: https://ror.org/03mwgfy56 Biotechnology Department, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran sug: ab: Liver zonation is a fundamental characteristic of hepatocyte spatial heterogeneity, which is challenging to recapitulate in traditional cell cultures. This study presents a novel microfluidic device designed to induce zonation in liver cell cultures by establishing an oxygen gradient using standard laboratory gases. The device consists of two layers; a bottom layer containing a gas channel network that delivers high (cell incubator air, 19% oxygen) and low oxygenated (nitrogen) gases to create three distinct zones within the cell culture chamber in the layer above. Computational simulations and ratiometric oxygen sensing were employed to validate the oxygen gradient, demonstrating that stable oxygen levels were achieved within two hours. Liver zonation was confirmed using immunofluorescence staining, which showed zonated albumin production in HepG2 cells directly correlating with oxygen levels and mimicking in-vivo zonation behavior. This user-friendly device supports studies on liver zonation and related metabolic disease mechanisms in vitro. It can also be utilized for experiments that necessitate precise gas concentration gradients, such as hypoxia-related research areas focused on angiogenesis and cancer development. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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