The activation of the purinergic system modulates the formation of foreign body giant cells in the presence of different metal alloys for clinical use.
Objective: two major complications after arthroplasty are prosthetic joint infection, mainly due to Staphylococcus aureus, and the experience of a foreign body reaction by macrophages and foreign body giant cells (FBGCs) regardless of the infection. Our aim is to study the role of purinergic recepto...
| Publicado en: | Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition) Vol. 16; no. 4; pp. 123 - 133 |
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| Autores principales: | , , , , , , , , , |
| Formato: | Artículo |
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Ibanez y Plaza
Oct-Dec2024
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=183300019&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 183300019 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 1889836X FQ8J jtl: Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition) issn: 1889836X maglogo: N pubinfo: dt: Oct-Dec2024 vid: 16 iid: 4 pid: 84111 pub: Ibanez y Plaza artinfo: ui: 183300019 10.20960/RevOsteoporosMetabMiner.00058 ppf: 123 ppct: 10 formats: fmt: @attributes: type: P size: 2.2MB tig: atl: The activation of the purinergic system modulates the formation of foreign body giant cells in the presence of different metal alloys for clinical use. aug: au: Jairo Aguilera-Correa, John Marco-Bonilla, Miguel Torrecilla-Sábada, Estíbaliz Conde, Ana Ángeles Arenas, María José de Damborenea, Juan Largo, Raquel Herrero-Beaumont, Gabriel Esteban, Jaime Mediero, Aránzazu affil: Clinical Microbiology Department. IIS-Fundación Jiménez Díaz. Universidad Autónoma de Madrid. Madrid, Spain. CIBERINFEC - Consorcio Centro de Investigación Biomédica en Red (CIBER) de Enfermedades Infecciosas. Madrid, Spain. Bone and Joint Research Unit. IIS-Fundación Jiménez Díaz. Universidad Autónoma de Madrid. Madrid, Spain. Department of Surface Engineering, Corrosion, and Durability. Centro Nacional de Investigaciones Metalúrgicas - CENIM. CSIC. Madrid, Spain. su: Prosthesis-related infections Gene expression Foreign body reaction Alloys Purinergic receptors Adenosines sug: subj: Prosthesis-related infections Gene expression Foreign body reaction Alloys Purinergic receptors Adenosines keyword: Adenosine. A2A infection Metal alloys. Prosthetic joint receptor. FBGCs. ab: Objective: two major complications after arthroplasty are prosthetic joint infection, mainly due to Staphylococcus aureus, and the experience of a foreign body reaction by macrophages and foreign body giant cells (FBGCs) regardless of the infection. Our aim is to study the role of purinergic receptors with fusogenic function (P2X7, adenosine A1 and A2A receptors) in the formation of S. aureus-induced FBGCs and their possible differential modulation in the presence of Ti–6Al–4V and Cr-Co-Mo alloys. Methods: RAW264.7 cells were differentiated to FBGCs with 20 ng/mL of IL-4 in the presence of adhered unviable S. aureus, metal alloys and/or CGS21680/ZM241385 1 µM. Cell supernatant was collected for nucleotide analysis by HPLC as well as cytokine expression, and cells were lysed for RNA expression. Results: the presence of S. aureus induces an increase in FBGCs formation in a concentration-dependent manner. Furthermore, phalloidin staining demonstrated that both Ti–6Al–4V and Cr-Co-Mo alloys reduce the formation of FBGCs. The expression of adenosine A1 and A2A receptors increased after 5 days of differentiation in the presence of S. aureus, and this expression was enhanced with metal alloys. HPLC analyses showed an increase in adenosine in the presence of Ti–6Al–4V and Cr-Co-Mo alloys while ATP was not changed in any of the conditions. The presence of metal alloys induced an increased in IL1β, IL-6 and RANTES. Conclusions: the increased levels of adenosine and the adenosine A2A receptor induced by the presence of Ti–6Al–4V and Co-Cr-Mo alloys would be responsible for the inhibition of cell fusion and the following reduction of S. aureus-induced FBGCs. pubtype: Academic Journal doctype: Article src: R language: Multiple languages refInfo: copyright: @attributes: flag: Y custom: Copyright of Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition) is the property of Ibanez y Plaza and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Journal of Osteoporosis & Mineral Metabolism / Revista de Osteoporosis y Metabolismo Mineral (Spanish edition) holder: Ibanez y Plaza dt: @attributes: year: 2024 holdings: @attributes: islocal: N |
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