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Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-Free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus.

Background & Objective: Colorectal cancer (CRC) is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus (PTCC 1643) supernatant (LAS) and lactobacillus...

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Published in:Journal of Advances in Medical & Biomedical Research Vol. 32; no. 155; pp. 449 - 458
Main Authors: Shokati, Ameneh, Soleimani, Masoud, Abroun, Saeid
Format: research tables/charts Journal Article
Published: Zanjan University of Medical Sciences & Health Services Nov/Dec2024
Online Access:View this record in EBSCOhost
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      issn: 26766264
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      dt: Nov/Dec2024
      vid: 32
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      pub: Zanjan University of Medical Sciences & Health Services
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        10.30699/jambr.32.155.449
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        atl: Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-Free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus.
      aug:
        au:
          Shokati, Ameneh
          Soleimani, Masoud
          Abroun, Saeid
        affil: Department of Hematology and Cell Therapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
      sug:
        subj:
          Colorectal Neoplasms Prevention and Control
          Lactobacillus Acidophilus
          Lactobacillus
          Antineoplastic Agents Pharmacodynamics
          Probiotics Pharmacodynamics
          Cell Line, Tumor Drug Effects
          Cell Proliferation Drug Effects
          Neoplasm Invasiveness Prevention and Control
          Neoplasm Metastasis Prevention and Control
          Human
          In Vitro Studies
          Immunohistochemistry Methods
          Reverse Transcriptase Polymerase Chain Reaction
          Cell Culture Techniques
          Gene Expression Drug Effects
          Mitochondrial Membranes Drug Effects
          Matrix Metalloproteinases Drug Effects
          Cell Death Drug Effects
          United States
          Cell Physiology Drug Effects
      ab: Background & Objective: Colorectal cancer (CRC) is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus (PTCC 1643) supernatant (LAS) and lactobacillus rhamnosus (PTCC 1657) supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro. Materials & Methods: In this study, the antiproliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chamber assays, respectively. Furthermore, the expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR. Results: The results of the MTT assay indicated that LAS and LRS had cytotoxic effects on Caco-2 cell proliferation at a concentration of 25% and higher after 72 hours (p<0.0001). Thus, the minimum concentrations (25%) of supernatants were chosen for further experiments. LRS and LAS could significantly suppress the invasiveness of cells, p= 0.028 and p=0.002, respectively. Also, the expression of MMP12 was significantly increased in cells when treated with LAS (p<0.001), whereas LRS had no significant effect on MMP-12 expression. Furthermore, the expression of MMP-9 was statistically decreased in cells treated with both supernatants (p<0.00001). Conclusion: In general, it was shown that LAS and LRS exert anti-cancer activity against the growth, invasion, and metastasis of Caco2 cells. These two bacteria could be used as prophylactic and therapeutic agents for the prevention and treatment of CRC.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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