ZEB1 expression in Th17 cells correlated with p-STAT3 in human apical periodontitis.
Background: ZEB1, a zinc-finger E homeobox-binding transcription factor most frequently associated with developmental programs linked to epithelial-mesenchymal transition, has been demonstrated to regulate immune cell function. The study aimed to investigate the expression pattern of ZEB1 in Th17 ce...
| Publicado en: | BMC Oral Health Vol. 25; no. 1; pp. 1 - 11 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
2/27/2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=183352289&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 183352289 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14726831 1CIC jtl: BMC Oral Health issn: 14726831 maglogo: N pubinfo: dt: 2/27/2025 vid: 25 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 183352289 183352289 183352289 10.1186/s12903-025-05633-y 183352289 ppf: 1 ppct: 10 formats: tig: atl: ZEB1 expression in Th17 cells correlated with p-STAT3 in human apical periodontitis. aug: au: Sun, Xiaoyue Yang, Jingwen Wang, Zijun Nie, Qing Yang, Qian Zhang, Wei Liu, Mingwen Wang, Li Zhu, Lingxin affil: https://ror.org/033vjfk17 State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, 430079, Wuhan, China sug: subj: DNA-Binding Proteins Transcription Factors Gene Expression T Lymphocytes STAT Proteins Periodontitis Funding Source Human Case Control Studies Inflammation Granuloma Odontogenic Cysts Staining and Labeling Immunohistochemistry Male Female Adult Middle Age Descriptive Statistics Unpaired T-Tests One-Way Analysis of Variance Data Analysis Software Interleukins Signal Transduction Fluorescent Antibody Technique Adult: 19-44 years Middle Aged: 45-64 years Male Female ab: Background: ZEB1, a zinc-finger E homeobox-binding transcription factor most frequently associated with developmental programs linked to epithelial-mesenchymal transition, has been demonstrated to regulate immune cell function. The study aimed to investigate the expression pattern of ZEB1 in Th17 cells and its colocalization with p-STAT3 in human apical periodontitis lesions. Methods: Thirty-nine human periapical tissues were collected for ex vivo study, including periapical granulomas (PGs, n = 14), radicular cysts (RCs, n = 12), and healthy control tissues (control group, n = 13). Inflammatory infiltration of the lesions was assessed using hematoxylin-eosin staining. The expression of ZEB1 was detected and analyzed by immunohistochemistry. The localization of ZEB1 in Th17 cells and its colocalization with p-STAT3 were assessed using fluorescence colocalization. Results: ZEB1 expression was significantly higher in PGs and RCs than in the healthy control group; however no significant difference between the two groups was observed. Immunofluorescence analysis revealed that ZEB1 expression was correlated with IL17 and CD4 double-positive cells in human periapical lesions. ZEB1/ p-STAT3 double-positive cells were predominant in RCs and PGs than in the healthy control group. Conclusions: The expression of ZEB1 was significantly elevated in PGs and RCs, and correlated with Th17 cells and p-STAT3 expression. This study revealed that ZEB1 is a potential player correlated with STAT3 activation and Th17 cells in apical periodontitis pathogenesis. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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