D1 dopamine / mu opioid receptor interactions in operant conditioning assays of pain-depressed responding and drug-induced rate suppression, and a conditioned place preference procedure: assessment of therapeutic index in male Sprague Dawley rats

Rationale and objectives: In vivo receptor interactions vary as a function of behavioral endpoint, with key differences between reflexive and non-reflexive measures that assess the motivational aspects of pain and pain relief. There have been no assessments of D1 dopamine agonist / mu opioid recepto...

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Published in:Psychopharmacology Vol. 242; no. 4; pp. 751 - 763
Main Authors: LaCourse, Hannah, Bennett, Lily, Falstad, April, Asmus, Francesca, Smith, Meghan, Davis, Ravin, Harrington, Kylee, Giuvelis, Denise, King, Tamara, Stevenson, Glenn W.
Format: Journal Article
Published: Springer Nature Apr2025
Online Access:View this record in EBSCOhost
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      dt: Apr2025
      vid: 242
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      pub: Springer Nature
      place: New York, New York
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        182316636
        10.1007/s00213-025-06743-9
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        atl: D1 dopamine / mu opioid receptor interactions in operant conditioning assays of pain-depressed responding and drug-induced rate suppression, and a conditioned place preference procedure: assessment of therapeutic index in male Sprague Dawley rats
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        au:
          LaCourse, Hannah
          Bennett, Lily
          Falstad, April
          Asmus, Francesca
          Smith, Meghan
          Davis, Ravin
          Harrington, Kylee
          Giuvelis, Denise
          King, Tamara
          Stevenson, Glenn W.
        affil: https://ror.org/02n2ava60 Department of Psychology, University of New England, Biddeford, ME, USA
      sug:
      ab: Rationale and objectives: In vivo receptor interactions vary as a function of behavioral endpoint, with key differences between reflexive and non-reflexive measures that assess the motivational aspects of pain and pain relief. There have been no assessments of D1 dopamine agonist / mu opioid receptor (MOR) agonist interactions in non-reflexive behavioral measures of pain. We examined the hypothesis that D1/MOR mixtures show enhanced effectiveness in blocking pain depressed behaviors while showing decreased side effects such as sedation and drug reward. Methods: SKF82958 and methadone were used as selective/high efficacy D1 and mu agonists, respectively. An FR10 operant schedule was utilized in the presence and absence of a lactic acid inflammatory pain-like manipulation, to measure antinociceptive and operant-rate-suppressing effects, respectively. Rewarding properties of the drug combinations were determined using a conditioned place preference procedure. Results: Methadone alone, but not SKF82958 alone, produced dose-dependent restoration of pain-depressed responding. Both SKF82958 and methadone produced dose-dependent response rate suppression. Three fixed proportion mixtures, based on the relative potencies of the drugs in the rate suppression assay, produced dose-dependent antinociception and sedation. Isobolographic analysis indicated that the 0.17:1 mixture produced supra-additive antinociception and additive sedation. The 0.055:1 mixture produced additive antinociception with sub-additive sedation, and the 0.018:1 mixture had the highest therapeutic index (TI) relative to other mixtures and drugs alone. The antinociceptive doses and component doses for the 0.018:1 mixture did not produce conditioned place preference. Conclusions: These results suggest that D1-selective dopamine agonists may have utility as candidate opioid-sparing analgesics.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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