Evaluation of plasma phytosterols in sitosterolemia, their kindreds and hyperlipidemia subjects.

• Mild phytosterol disruption in sitosterolemia kindred with single mutation. • A discriminative role of 7-dehydrocholesterol reductase in distinguishing hyperlipidemia and sitosterolemia kindred. • The combination of 6 phytosterols proved value than clinical lipid index in distinguishing between si...

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Publicado en:Journal of Clinical Lipidology Vol. 19; no. 1; pp. 146 - 156
Autores principales: Ren, Xuanru, Zhang, Jun, Wang, Luya, Zhang, Yuxuan, Li, Jialu, Yu, Hao, Zheng, Zhaohai, Zhang, Yiqing, Zeng, Hesong, Chen, Yan, Wu, Junfang
Formato: research Journal Article
Publicado: Elsevier B.V. Jan2025
Acceso en línea:Ver este registro en EBSCOhost
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        19332874
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      jtl: Journal of Clinical Lipidology
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      dt: Jan2025
      vid: 19
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      pub: Elsevier B.V.
      place: New York, New York
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        183600077
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        10.1016/j.jacl.2024.09.002
        183600077
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        atl: Evaluation of plasma phytosterols in sitosterolemia, their kindreds and hyperlipidemia subjects.
      aug:
        au:
          Ren, Xuanru
          Zhang, Jun
          Wang, Luya
          Zhang, Yuxuan
          Li, Jialu
          Yu, Hao
          Zheng, Zhaohai
          Zhang, Yiqing
          Zeng, Hesong
          Chen, Yan
          Wu, Junfang
        affil: Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
      sug:
        subj:
          Phytosterols Blood
          Metabolism, Inborn Errors
          Hyperlipidemia
          Mutation
          Human
          Retrospective Design
          Cholesterol Metabolism
          Biological Markers
      ab: • Mild phytosterol disruption in sitosterolemia kindred with single mutation. • A discriminative role of 7-dehydrocholesterol reductase in distinguishing hyperlipidemia and sitosterolemia kindred. • The combination of 6 phytosterols proved value than clinical lipid index in distinguishing between sitosterolemia, its single mutation kindred, and hyperlipidemia. Patients suffering from sitosterolemia with ABCG5/8 mutation typically present with early-onset or rapidly progressive atherosclerosis. Their kindreds with partial genetic deficiencies of ABCG5/8 are often considered healthy. However, discerning sitosterolemia from its familial kindreds and hyperlipidemia subjects has remained challenging. Here we retrospectively recruited 7 families including 8 individuals diagnosed with sitosterolemia subjects, and 14 kindreds carrying single gene mutations. Additionally, 17 individuals with hyperlipidemia and 130 healthy controls served as positive and negative controls, respectively. A total of 6 phytosterols combined with cholesterol absorption indices (including sitosterol, campesterol, stigmasterol, and cholestanol) and cholesterol synthesis markers (desmosterol and 7-dehydrocholesterol), was compared across the aforementioned 4 groups. As expected, the sitosterolemia subjects with double mutations demonstrated significantly elevated levels of sitosterol and other cholesterol absorption indices. Meanwhile, sitosterolemia kindreds with single gene mutation showed a similar pattern of activated cholesterol-absorption ability to the hyperlipidemia group, but not as high as the double mutation group. Notably, the cholesterol-synthesis enzyme 7-dehydrocholesterol reductase displayed an increase in the hyperlipidemia group but a decrease in the sitosterolemia kindred group, suggesting a potential discriminative role of 7-dehydrocholesterol in distinguishing between these 2 groups. The combination of phytosterols was more valuable than clinical lipid index for sitosterolemia diagnosis. Our study revealed mild disruptions of cholesterol absorption capacities in sitosterolemia kindreds with single mutations. Furthermore, the combination of 6 phytosterols proved effective in distinguishing between sitosterolemia, its single mutation carriers, and hyperlipidemia patients. [Display omitted]
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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