Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer.
Background: Aberrant methylation and expression of various noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), confer a great potential as tumor markers. This study aimed to investigate miR-30b DNA methylation and metastasis associated lung adenocarcinoma transcript 1 (MA...
| Published in: | Middle East Journal of Cancer Vol. 16; no. 2; pp. 127 - 139 |
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| Main Authors: | , , , , , , , |
| Format: | research tables/charts Journal Article |
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Middle East Journal of Cancer
Apr2025
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=184213855&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 184213855 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20086709 B7LE jtl: Middle East Journal of Cancer issn: 20086709 maglogo: N pubinfo: dt: Apr2025 vid: 16 iid: 2 pid: 67247 pub: Middle East Journal of Cancer artinfo: ui: 184213855 184213855 184213855 10.30476/mejc.2024.101524.2035 184213855 ppf: 127 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer. aug: au: Memarzadeh, Marziyeh Zarredar, Habib Asadi, Milad Sadeghi, Armin Zafari, Venus Hashemzadeh, Shahryar Sabbagh-Jadid, Hamed Raeisi, Mortaza affil: Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran sug: subj: Carcinoma, Non-Small-Cell Lung Diagnosis DNA Methylation Evaluation MicroRNA Metabolism RNA Metabolism Biological Markers Analysis Human Cross Sectional Studies Genomics DNA Analysis Polymerase Chain Reaction Methods RNA Analysis Data Analysis Software Carcinoma, Non-Small-Cell Lung Metabolism Lymph Nodes Pathology Carcinoma, Non-Small-Cell Lung Physiopathology ROC Curve Funding Source ab: Background: Aberrant methylation and expression of various noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), confer a great potential as tumor markers. This study aimed to investigate miR-30b DNA methylation and metastasis associated lung adenocarcinoma transcript 1 (MALAT-1) expression patterns as potential diagnostic biomarkers for non-small cell lung cancer (NSCLC). Method: In this cross-sectional study, miR-30b DNA methylation and MALAT- 1 expression patterns were first explored using microarray data retrieved from the NSCLC dataset in the Cancer Genome Atlas (TCGA)-LUNG. Then, the obtained results were further validated in internal samples. Subsequently, genomic DNA was extracted and modified by sodium bisulfite to determine DNA methylation using q- MSP. Total RNA was extracted and transcribed to cDNA to measure transcription level by quantitative real-time polymerase chain reaction. GraphPad 6 Prism v.8 was used to perform the statistical analyses. Comparisons between groups in internal samples were conducted by paired student's t-test, while Mann-Whitney U test was used to analyze TCGA-LUNG data (P < 0.05). Results: Our results indicated miR-30b hypermethylation, miR-30b downregulation and lncRNA MALAT-1 overexpression in NSCLC tumor samples compared with marginal normal samples. These changes were significantly associated with the stage of malignancy like lymph node metastasis. Also, using receiver operating characteristic curve analysis, MALAT-1 expression, and miR-30b methylation and expression patterns were found as possible diagnostic biomarkers for NSCLC (area under the curve was 0.70, 0.67, and 0.74, respectively). Conclusion: We found involvement of miR-30b hypermethylation and downregulation as well as lncRNA MALAT-1 overexpression with tumor outcomes of NSCLC patients. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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