Unveiling the therapeutic potential of HZQYF: exploring the inhibitory impact of a clinical herbal formula on gastric cancer through network pharmacology and transcript analysis.
Hezi Qingyou Formula (HZQYF) is a clinical formulation known for its efficacy in treating gastrointestinal diseases. Nevertheless, its specific impact and underlying mechanism of action in gastric cancer remain to be fully elucidated. The major components of the formula were precisely identified and...
| Publicado en: | BMC Complementary Medicine & Therapies Vol. 25; no. 1; pp. 1 - 14 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
4/17/2025
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=184555967&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 184555967 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 26627671 MFH8 jtl: BMC Complementary Medicine & Therapies issn: 26627671 maglogo: N pubinfo: dt: 4/17/2025 vid: 25 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 184555967 184555967 184555967 10.1186/s12906-025-04871-5 184555967 ppf: 1 ppct: 13 formats: tig: atl: Unveiling the therapeutic potential of HZQYF: exploring the inhibitory impact of a clinical herbal formula on gastric cancer through network pharmacology and transcript analysis. aug: au: Feng, Zhong Ou, Ling Li, Hui Hao, Yajie Wei, Ruixia Zhang, Guimin Yao, Meicun affil: https://ror.org/0064kty71 School of Pharmaceutical Sciences (Shenzhen), Sun Yat-Sen University, 518107, Shenzhen, China sug: subj: Drugs, Chinese Herbal Pharmacodynamics Stomach Neoplasms Drug Therapy Network Pharmacology Funding Source Human Chromatography, High Pressure Liquid Liquid Chromatography-Mass Spectrometry In Vitro Studies Cell Viability Drug Effects Cell Line, Tumor Drug Effects Reactive Oxygen Species Drug Effects Phytochemicals Analysis T-Tests Data Analysis Software Descriptive Statistics Signal Transduction Drug Effects Nitrogen Metabolism GABA Metabolism ab: Hezi Qingyou Formula (HZQYF) is a clinical formulation known for its efficacy in treating gastrointestinal diseases. Nevertheless, its specific impact and underlying mechanism of action in gastric cancer remain to be fully elucidated. The major components of the formula were precisely identified and characterized using ultra-high-performance liquid chromatography coupled with a tandem mass spectrometer (UHPLC-MS/MS). Network pharmacology and transcript analysis were utilized to identify the targets associated with drug-disease interactions. Subsequently, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome analyses were conducted to unravel the pivotal pathways involved. Furthermore, in vitro experiments were performed to validate the anti-gastric cancer activity of HZQYF, including assessments of cell viability and clonogenic potential. These results revealed that 260 co-expressed targets were identified as shared between HZQYF and gastric cancer. These genes were significantly enriched in biological processes and pathways related to steroid metabolism, gamma-aminobutyric acid (GABA)-A receptor complex, steroid binding activity, extracellular ligand-gated ion channel activity, chemical carcinogenesis-reactive oxygen species, and GABAergic synapse. Furthermore, the principal components of the formula were characterized. Subsequent cell experiments confirmed the formula's ability to inhibit gastric cancer activity and suppress colony formation in vitro. In conclusion, these findings suggest that Hezi Qingyou Formula may exert its anti-gastric cancer activity by influencing reactive oxygen species and modulating GABAergic synapses in-silico methods. This study provides a foundation for further exploration of HZQYF as a potential therapeutic agent for gastric cancer. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|