Effects of nocodazole and latrunculin B on locomotion of amoeboid cells of Rhizochromulina sp. strain B44 (Heterokontophyta, Dictyochophyceae).
Rhizochromulina is a genus of unicellular dictyochophycean algae (Heterokontophyta), comprising a single species R. marina and numerous strains. Recently, we described the first arctic rhizochromuline—Rhizochromulina sp. strain B44. Amoeboid cells of this algae are able to transform into flagellates...
| Publicado en: | Protoplasma Vol. 262; no. 3; pp. 585 - 595 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
May2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=184705676&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 184705676 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: May2025 vid: 262 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 184705676 181876608 10.1007/s00709-024-02024-y 184705676 ppf: 585 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Effects of nocodazole and latrunculin B on locomotion of amoeboid cells of Rhizochromulina sp. strain B44 (Heterokontophyta, Dictyochophyceae). aug: au: Safonov, Pavel Khaitov, Vadim Palii, Olga Skarlato, Sergei Berdieva, Mariia affil: https://ror.org/037styt87 Institute of Cytology, Russian Academy of Sciences, 194064, Saint Petersburg, Russia sug: ab: Rhizochromulina is a genus of unicellular dictyochophycean algae (Heterokontophyta), comprising a single species R. marina and numerous strains. Recently, we described the first arctic rhizochromuline—Rhizochromulina sp. strain B44. Amoeboid cells of this algae are able to transform into flagellates, and this transition can be triggered by prolonged mechanical disturbance. Thin branching pseudopodia of the neighboring rhizochromuline cells fuse to form a meroplasmodium. The pseudopodia contain microtubules, but do not contain actin microfilaments; actin forms the cytoplasmic cytoskeleton and extends only to the bases of the pseudopodia. Microtubule-driven pseudopodia are characteristic to a plethora of eukaryotes, but the role of microtubular and actin cytoskeleton in locomotion of these organisms remains poorly understood. We conducted a series of experiments where amoeboid cells of Rhizochromulina sp. B44 were treated with either 10 µM nocodazole, 10 µM latrunculin B, or both drugs simultaneously. Cellular locomotion was captured on camera, tracked, and then analyzed with the help of the generalized additive mixed model. The obtained results indicate that both drugs, when applied separately, decrease the motility of the studied cells. Unexpectedly, the combined treatment had the opposite effect, as the cells became more motile. The analysis also revealed a non-linear pattern of relationship between motility of amoeboid cells of rhizochromulines and density of their population. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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