Wenjing Zhitong Decoction Alleviates Primary Dysmenorrhea Mediated by Pain Sensitization Via the ERα‐BDNF Signaling Pathway.
Background: Wen‐Jing‐Zhi‐Tong decoction (WJZTD) is a traditional Chinese medicine herbal decoction that has demonstrated clinical efficacy in treating primary dysmenorrhea (PDM) over decades. However, the underlying therapeutic mechanism of WJZTD requires further clarification. Objective: This study...
| Publicado en: | Journal of Clinical Pharmacy & Therapeutics Vol. 2025; pp. 1 - 19 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
5/14/2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=185186361&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 185186361 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02694727 EV4 jtl: Journal of Clinical Pharmacy & Therapeutics issn: 02694727 maglogo: Y pubinfo: dt: 5/14/2025 vid: 2025 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 185186361 185186361 185186361 10.1155/jcpt/5516416 185186361 ppf: 1 ppct: 18 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Wenjing Zhitong Decoction Alleviates Primary Dysmenorrhea Mediated by Pain Sensitization Via the ERα‐BDNF Signaling Pathway. aug: au: Qin, Yajie Yang, Xiaotian Tang, Xingran Zhao, Huijin Zhou, Huifang Chauhan, Anjali affil: The First Clinical Medical College,, Nanjing University of Chinese Medicine,, Nanjing, Jiangsu, China, njucm.edu.cn sug: subj: Dysmenorrhea Drug Therapy Medicine, Chinese Traditional Drugs, Chinese Herbal Therapeutic Use Drugs, Chinese Herbal Pharmacodynamics Drug Efficacy Evaluation Metabolomics Receptors, Estrogen Brain-Derived Neurotrophic Factor Signal Transduction Animal Studies Rats Funding Source Network Pharmacology In Vitro Studies In Vivo Studies Prostaglandins Blood Fluorescent Antibody Technique Blotting, Western Ganglia, Sensory ab: Background: Wen‐Jing‐Zhi‐Tong decoction (WJZTD) is a traditional Chinese medicine herbal decoction that has demonstrated clinical efficacy in treating primary dysmenorrhea (PDM) over decades. However, the underlying therapeutic mechanism of WJZTD requires further clarification. Objective: This study aims to explore and elucidate the therapeutic mechanism of WJZTD in treating PDM through metabolomics, integrated network pharmacology, as well as in vitro and in vivo experiments. Methods: A PDM rat model was established by estradiol benzoate and oxytocin for 3 consecutive estrous cycles, and then WJZTD treatment was administered for 3 consecutive estrous cycles. To evaluate the therapeutic effect of WJZTD on PDM, behavioral tests, histological analysis, and evaluation of serum prostaglandins (PGs) were performed. Moreover, immunofluorescence and Western blot analysis were carried out in dorsal root ganglion (DRG) tissues to explore the role of WJZTD in reducing pain sensitivity. Metabolomics and integrated pharmacology were utilized to identify potential bioactive targets, which were then validated through in vitro experiments. Results: WJZTD significantly reduces pain sensitivity in PDM rats, treating the condition effectively by decreasing brain‐derived neurotrophic factor (BDNF) protein expression, c‐Fos‐positive neurons in DRG, and serum prostaglandin PGF2α (PGF2α) levels. A nontargeted metabolic analysis identified 11 differential metabolites, suggesting ESR1 (estrogen receptor α, ERα) as a potential target. In vitro experiments confirmed WJZTD's efficacy in treating PDM through regulating the ERα/BDNF signaling pathway. Conclusion: WJZTD decreases the excitability of DRG neurons in rats with PDM through the ERα/BDNF pathway, which enhances pain sensitization and contributes to reducing dysmenorrhea. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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