Nuclear paraspeckle assembly transcript 1 promotes photophobia behavior in mice via miR-196a-5p/Trpm3 coupling.
Background: The long noncoding RNA, NEAT1, is recognized as a key regulator of proinflammatory gene expression; Yet, its functional role in migraine remains unexplored, despite the central role of neuroinflammatory mechanisms in migraine pathophysiology. This study examines the implication of NEAT1...
| Publicado en: | Journal of Headache & Pain Vol. 26; no. 1; pp. 1 - 16 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
5/22/2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=185446356&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 185446356 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11292369 O3T jtl: Journal of Headache & Pain issn: 11292369 maglogo: N pubinfo: dt: 5/22/2025 vid: 26 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 185446356 185446356 185446356 10.1186/s10194-025-02057-5 185446356 ppf: 1 ppct: 15 formats: tig: atl: Nuclear paraspeckle assembly transcript 1 promotes photophobia behavior in mice via miR-196a-5p/Trpm3 coupling. aug: au: Huang, Zhuoan Li, Xingshen Wang, Xiaolin Wu, Jiaqi Gong, Ziyang Kõks, Sulev Wang, Minyan affil: https://ror.org/03zmrmn05 Department of Biosciences and Bioinformatics, School of Science, Centre for Neuroscience, Xi'an Jiaotong-Liverpool University (XJTLU), 215123, Suzhou, China sug: subj: RNA Physiology Ganglia, Sensory Physiology Photophobia Physiopathology Migraine Physiopathology MicroRNA Physiology Carrier Proteins Physiology Animal Studies Behavior, Animal Light Ketones Administration and Dosage Administration, Intranasal Luminescent Measurements Mice Gene Expression Evaluation RNA Analysis Sequence Analysis Methods Polymerase Chain Reaction Evaluation Histology RNA Administration and Dosage Injections, Intravenous Genetic Techniques Photophobia Prevention and Control MicroRNA Analysis Ganglia, Sensory Metabolism Carrier Proteins Analysis Funding Source ab: Background: The long noncoding RNA, NEAT1, is recognized as a key regulator of proinflammatory gene expression; Yet, its functional role in migraine remains unexplored, despite the central role of neuroinflammatory mechanisms in migraine pathophysiology. This study examines the implication of NEAT1 in the trigeminal ganglion activation, which underlies photophobia associated with migraine. Methods: Light aversion behavior was induced by intranasal injection of the TRPA1 activator, umbellulone. Male mouse behavior was assessed by the total time the mouse stays in the light between the dark and light compartments. To gain insight to the NEAT1-mediated photophobia mechanism, gene expression of candidate genes and non-coding RNAs interactions were assessed using RNA-sequencing, qPCR analysis, histology and dual-luciferase reporter gene assay. Results: NEAT1 was upregulated in the trigeminal ganglion of male photophobia mice; Downregulation of NEAT1 by intravenous injection of shNEAT1 adeno-associated virus vectors attenuated NEAT1 expression and alleviated photophobia-like behavior in mice. The elevated NEAT1 expression in the trigeminal ganglion of photophobia mice corresponds to the downregulation of miR-196a-5p and upregulation Trpm3 RNA level. Predicted analysis suggested NEAT1/miR-196a-5p ceRNA network exists in photophobia mice. Indeed, knocking down NEAT1 upregulated miR-196a-5p, whilst downregulated Trpm3 gene expression level, in the trigeminal ganglion of photophobia mice. Further investigation using dual-luciferase reporter gene assay identified NEAT1 interacting with miR-196a-5p, whilst miR-196a-5p interacting with Trpm3. Similar to knocking down NEAT1, TRPM3 inhibition reduced photophobia-like behavior. Conclusion: We conclude that NEAT1 is critical for promoting photophobia behavior via miR-196a-5p/Trpm3 coupling. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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