Infants < 90 days of age with late-onset sepsis display disturbances of the microbiome-immunity interplay.
Objective: We hypothesized that previously healthy infants < 90 days of age with late-onset sepsis (LOS) have disturbances of the gut microbiome with yet undefined specific immunological patterns. Methods: We performed a prospective single-center convenience sample study between January 2019 and Jul...
| Published in: | Infection Vol. 53; no. 3; pp. 921 - 935 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Springer Nature
Jun2025
|
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=185686802&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 185686802 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Jun2025 vid: 53 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 185686802 180873306 185686802 185686802 10.1007/s15010-024-02396-6 185686802 ppf: 921 ppct: 14 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Infants < 90 days of age with late-onset sepsis display disturbances of the microbiome-immunity interplay. aug: au: Graspeuntner, Simon Lupatsii, Mariia van Zandbergen, Vera Dammann, Marie-Theres Pagel, Julia Nguyen, Duc Ninh Humberg, Alexander Göpel, Wolfgang Herting, Egbert Rupp, Jan Härtel, Christoph Fortmann, Ingmar affil: https://ror.org/00t3r8h32 Department of Infectious Diseases and Microbiology, University of Lübeck, Lübeck, Germany sug: subj: Sepsis Microbiology Sepsis Immunology Age of Onset Gut Microbiota Immunology T-Lymphocytes, Regulatory Immunology Human Funding Source Male Female Infant Prospective Studies Case Control Studies Comparative Studies Descriptive Statistics Convenience Sample Lymphocytes Analysis Feces Microbiology Bifidobacterium Immunity CD4 Lymphocyte Count Infant: 1-23 months Male Female ab: Objective: We hypothesized that previously healthy infants < 90 days of age with late-onset sepsis (LOS) have disturbances of the gut microbiome with yet undefined specific immunological patterns. Methods: We performed a prospective single-center convenience sample study between January 2019 and July 2021 in a case-control design. Routine diagnostics included conventional cultures (blood, cerebrospinal fluid, urine), PCRs and inflammatory markers in infants aged < 90 days with clinical LOS. We additionally analyzed blood lymphocyte subsets including CD4 + CD25 + forkhead box protein (FoxP3)+ Tregs and performed 16 S rRNA sequencing of stool samples, both compared to age-matched healthy controls. Results were adjusted for potential confounders that may influence microbial composition. Results: 51 infants with fever and clinical LOS were enrolled. Bacterial sepsis was diagnosed in n = 24 (47.1%) and viral infection in n = 13 (25.5%) infants, whereas in 14 (27.3%) infants the cause of fever remained undetermined. When compared to healthy controls, the gut microbiome of LOS infants at disease onset was characterized by a shift in community composition, specifically, decreased abundance of B. longum and an increase of Bacteroidia spp. Intriguingly, the abundance of B. longum negatively correlated with the frequency of blood CD4-positive cells in healthy controls but not in infants with LOS. At one year of age, we observed microbiome differences in infants with history of LOS when compared to healthy controls, such as an increased gut microbial diversity. Conclusion: Our data suggest potential signatures of the microbiome-immunity interplay in infants with LOS, which should be investigated further as possible targets for prevention. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|