Targeted next-generation sequencing - a promising approach in the diagnosis of Mycobacterium tuberculosis and drug resistance.
Targeted next-generation sequencing (tNGS) offers a high-throughput, culture-independent approach that delivers a comprehensive resistance profile in a significantly shorter turn-around time, making it promising in enhancing tuberculosis (TB) diagnosis and informing treatment decisions. This study a...
| Published in: | Infection Vol. 53; no. 3; pp. 967 - 980 |
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| Main Authors: | , , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Springer Nature
Jun2025
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=185686807&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 185686807 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Jun2025 vid: 53 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 185686807 180315803 185686807 185686807 10.1007/s15010-024-02411-w 185686807 ppf: 967 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Targeted next-generation sequencing - a promising approach in the diagnosis of Mycobacterium tuberculosis and drug resistance. aug: au: Wu, Xiaocui Tan, Guangkun Sun, Chunlei Wang, Yang Yang, Jinghui Wu, Chunqiu Hu, Chaohui Yu, Fangyou affil: https://ror.org/03rc6as71 Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China sug: subj: Sequence Analysis Methods Tuberculosis Diagnosis Drug Resistance, Microbial Evaluation Mutation Evaluation Phenotype Funding Source Human Academic Medical Centers Bronchoalveolar Lavage Microbial Culture and Sensitivity Tests Descriptive Statistics Tuberculosis, Pulmonary Diagnosis Genomics Bioinformatics Polymerase Chain Reaction Sensitivity and Specificity Confidence Intervals Predictive Value of Tests Data Analysis Software Amikacin Isoniazid Kanamycin Rifampin Ethambutol False Positive Results Male Female Adolescence Adult Middle Age Aged Aged, 80 and Over Adolescent: 13-18 years Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Aged, 80 & over Male Female ab: Targeted next-generation sequencing (tNGS) offers a high-throughput, culture-independent approach that delivers a comprehensive resistance profile in a significantly shorter turn-around time, making it promising in enhancing tuberculosis (TB) diagnosis and informing treatment decisions. This study aims to evaluate the performance of tNGS in the TB diagnosis and drug resistance detection of Mycobacterium tuberculosis (MTB) using MTB clinical isolates and bronchoalveolar lavage fluid (BALF) samples. A total of 143 MTB clinical isolates were assessed, tNGS, phenotypic antimicrobial susceptibility testing (AST), and AST based on whole genome sequencing (WGS) exhibited high concordance rates, averaging 95.10% and 97.05%. Among 158 BALF samples, culture, Xpert MTB/RIF, and tNGS reported 29, 70 and 111 positives, respectively. In the confirmed cases with etiological evidence (smears, cultures, or molecular test), the positive rate of tNGS (73/83, 87.95%) was higher than that of Xpert MTB (67/83, 80.72%). Additionally, 45% (27/60) of clinically diagnosed cases (with imaging or immunological evidence) were positive for tNGS. Further validation on the discrepant results between tNGS and Xpert MTB/RIF with droplet digital PCR (ddPCR) yielded 35 positives, tNGS detected all, and Xpert MTB/RIF only identified 6 positives. In conclusion, tNGS demonstrates robust and rapid performance in the identification of MTB and its associated drug resistance, and can be directly applied to clinical samples, positioning it as a promising approach for laboratory testing of tuberculosis. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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