Lab on chips for liquid biopsy: a flexible and customized approach through microfabrication.
Cancer early detection is one of the most challenging purposes of preventive medicine. Liquid biopsy represents a revolutionary approach, fostering access to early screening and increasing patients’ compliance, two crucial issues in reaching the largest possible audience in prevention campaigns. To...
| Publicado en: | Biomedical Microdevices Vol. 27; no. 2; pp. 1 - 13 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jun2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=185796827&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 185796827 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Jun2025 vid: 27 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 185796827 10.1007/s10544-025-00757-y 185796827 ppf: 1 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Lab on chips for liquid biopsy: a flexible and customized approach through microfabrication. aug: au: Garzarelli, Valeria Foscarini, Alessia Indirli, Vanessa Menon, Ilaria Mangiullo, Diego Verri, Tiziano Primiceri, Elisabetta Nigro, Annamaria Quattrini, Angelo Romano, Alessandro Chiriacò, Maria Serena Gigli, Giuseppe Ferrara, Francesco affil: https://ror.org/0042e5975 CNR NANOTEC– Institute of Nanotechnology, via per Monteroni, 73100, Lecce, Italy sug: ab: Cancer early detection is one of the most challenging purposes of preventive medicine. Liquid biopsy represents a revolutionary approach, fostering access to early screening and increasing patients’ compliance, two crucial issues in reaching the largest possible audience in prevention campaigns. To facilitate this approach, the deployment of innovative methods for easy manipulation of biological fluids and the availability of devices for the rapid and low-cost detection of biomarkers is essential. The aim of this study was the optimization of multifunctional Lab-On-Chips with the final aim of realizing a platform for oral carcinoma cells trapping from a complex biological fluid as saliva and for specific subcellular components like extracellular vesicles (EVs) from the neuroblastoma cell model. A set of different microfluidic building blocks was realized through poly-methyl methacrylate (PMMA) micromilling, microfabricated and functionalized to optimize surface chemistry for capturing tumor cells or EVs in multiple channels, assess working concentration for biological fluids and combine sample preparation with detection modules all in the same chip. After optimization, a proof-of-concept device was realized mimicking liquid biopsy analysis from saliva, a biological fluid readily available and with a high compliance from patients, useful for the early diagnosis of cancer. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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