Sequencing-Based Detection of Measles in Wastewater: Texas, January 2025.
Measles is a potentially deadly viral infection spread via respiratory droplets from infected individuals. Outbreaks occur when vaccine coverage drops below the threshold of herd, or community, immunity. Using a sequencing-based approach, we report the prospective (January 7, 2025) detection of meas...
| Publicado en: | American Journal of Public Health Vol. 115; no. 7; pp. 1115 - 1120 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | Artículo |
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American Public Health Association
Jul2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ssf&AN=185864042&site=ehost-live header: @attributes: shortDbName: ssf uiTerm: 185864042 longDbName: Social Sciences Full Text (H.W. Wilson) uiTag: AN controlInfo: bkinfo: jinfo: jid: 00900036 APH jtl: American Journal of Public Health issn: 00900036 maglogo: N pubinfo: dt: Jul2025 vid: 115 iid: 7 pid: 44 pub: American Public Health Association artinfo: ui: 185864042 10.2105/AJPH.2025.308146 ppf: 1115 ppct: 5 formats: tig: atl: Sequencing-Based Detection of Measles in Wastewater: Texas, January 2025. aug: au: Javornik Cregeen, Sara Tisza, Michael J. Hanson, Blake Cook, Marissa Surathu, Anil Schneider, Rebecca Wu, Jingjing Short, Kirstin Domakonda, Kaavya Hopkins, Loren Ross, Matthew C. Petrosino, Joseph F. Deegan, Jennifer Stadler, Lauren B. Boerwinkle, Eric Maresso, Anthony affil: Alkek Center for Metagenomics and Microbiome Research and the Department of Molecular Virology and Microbiology at Baylor College of Medicine, Houston, TX. School of Public Health at University of Texas Health Science Center at Houston. Houston Health Department. Department of Civil and Environmental Engineering at Rice University, Houston. Houston Health Department, Rice University, Houston. su: Texas Public health surveillance Cost effectiveness Epidemics Water pollution Sewage analysis Environmental monitoring Research funding Measles Polymerase chain reaction Aquatic microbiology Longitudinal method Nucleic acids Sequence analysis Genotypes Genomes Vaccination status Sensitivity & specificity (Statistics) sug: subj: Public health surveillance Cost effectiveness Epidemics Water pollution Texas Environmental Consulting Services Sewage analysis Environmental monitoring Research funding Measles Polymerase chain reaction Aquatic microbiology Longitudinal method Nucleic acids Sequence analysis Genotypes Genomes Vaccination status Sensitivity & specificity (Statistics) ab: Measles is a potentially deadly viral infection spread via respiratory droplets from infected individuals. Outbreaks occur when vaccine coverage drops below the threshold of herd, or community, immunity. Using a sequencing-based approach, we report the prospective (January 7, 2025) detection of measles in nucleic acid extracts from 2 wastewater treatment plants in Houston, Texas, with a population of more than 218 000 residents. The sequencing data from 2 samples contained 53 unique reads mapping to 11 different regions of the measles virus genome with a 99.4% match to genotype B3. Importantly, no detections were observed from 821 previous samples from the same city spanning nearly 3 years of monitoring. The findings were confirmed using droplet digital polymerase chain reaction. A concomitant investigation identified 2 unvaccinated measles-positive travelers living within the same sewershed as the wastewater detection event. This work suggests that sequencing-based wastewater analysis is valuable as a comprehensive early detection warning system that facilitates more targeted epidemiological investigation. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: N holdings: @attributes: islocal: N |
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