A Modified Ligature‐Induced Peri‐Implantitis Murine Model and RNA Sequencing Analysis Compared With Human Subjects.
Aim: To propose a modified method for establishing a peri‐implantitis murine model, assess the gene expression profile and immune cell infiltration of the gingiva and alveolar bone, and evaluate the transcriptomic similarity between patients with peri‐implantitis and the corresponding murine model....
| Publicado en: | Journal of Clinical Periodontology Vol. 52; no. 5; pp. 787 - 799 |
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| Autores principales: | , , , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
May2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=186111968&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 186111968 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03036979 8E3 jtl: Journal of Clinical Periodontology issn: 03036979 maglogo: Y pubinfo: dt: May2025 vid: 52 iid: 5 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 186111968 183793852 186111968 186111968 10.1111/jcpe.14127 186111968 ppf: 787 ppct: 12 formats: tig: atl: A Modified Ligature‐Induced Peri‐Implantitis Murine Model and RNA Sequencing Analysis Compared With Human Subjects. aug: au: Tong, Zi‐an Chen, Long Shen, Ling Lu, Yi‐fan Zhang, Jian‐wei Qi, Ya‐dong Qian, Yin‐jie Bao, Si‐qi Chen, Wei Si, Mi‐si affil: Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou Zhejiang,, China sug: subj: Peri-Implantitis Familial and Genetic Peri-Implantitis Immunology Gingiva Pathology Alveolar Process Pathology Gene Expression Profiling Sequence Analysis Genome Wide Association Study Histological Techniques Disease Progression Animal Studies Mice Funding Source Human Comparative Studies Macrophages Neutrophils Peri-Implantitis Etiology Microscopy, Electron, Scanning Maxilla Pathology Biopsy Tomography, X-Ray Computed Immunohistochemistry Fluorescent Antibody Technique Polymerase Chain Reaction Bioinformatics Unpaired T-Tests Pearson's Correlation Coefficient Descriptive Statistics Data Analysis Software Immune System ab: Aim: To propose a modified method for establishing a peri‐implantitis murine model, assess the gene expression profile and immune cell infiltration of the gingiva and alveolar bone, and evaluate the transcriptomic similarity between patients with peri‐implantitis and the corresponding murine model. Materials and Methods: A ligature‐induced peri‐implantitis murine model was established using an immediate implant placement approach. RNA sequencing was performed to determine the transcriptomic profiles of peri‐implant tissues from mice, patients with peri‐implantitis and healthy individuals. Histopathological and bioinformatics analyses were performed to measure immune cell infiltration, bone remodelling, and inflammatory reactions. Results: Mouse gingival tissue showed strong immune and inflammatory responses, especially macrophage functions; these responses were weaker in the alveolar bone. Humans and mice showed similar gene expression patterns in the gingiva, with greater infiltration of macrophages and neutrophils. Conclusions: During peri‐implantitis progression, mouse gingival tissue exhibited increased immune‐related functions and inflammation compared with the alveolar bone. Patients with peri‐implantitis and the murine model displayed transcriptomic similarities within the gingiva. We propose that the modified ligature‐induced peri‐implantitis murine model is suitable for investigating peri‐implantitis pathogenesis, with macrophages and neutrophils potentially being critical in its development. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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