Distribution of endocannabinoid system receptors in the equine hoof: dysregulation as a potential therapeutic target for laminitis.

A growing body of evidence indicates that the endocannabinoid system (ECS) is essential for controlling the homeostasis of the skin and that the ECS is modified in the presence of skin disease. It is plausible to expect that the lamellar junction of the hoof expresses cannabinoid receptors and that...

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Publicado en:Histochemistry & Cell Biology Vol. 163; no. 1; pp. 1 - 17
Autores principales: Zamith Cunha, Rodrigo, Gobbo, Francesca, Morini, Maria, Zannoni, Augusta, Mainardi, Carlo, D'arpe, Lorenzo, Gramenzi, Alessandro, Chiocchetti, Roberto
Formato: Journal Article
Publicado: Springer Nature 7/1/2025
Acceso en línea:Ver este registro en EBSCOhost
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      place: New York, New York
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        atl: Distribution of endocannabinoid system receptors in the equine hoof: dysregulation as a potential therapeutic target for laminitis.
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          Zamith Cunha, Rodrigo
          Gobbo, Francesca
          Morini, Maria
          Zannoni, Augusta
          Mainardi, Carlo
          D'arpe, Lorenzo
          Gramenzi, Alessandro
          Chiocchetti, Roberto
        affil: https://ror.org/01111rn36 Department of Veterinary Medical Sciences (UNI EN ISO 9001:2008), University of Bologna, Bologna, Italy
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      ab: A growing body of evidence indicates that the endocannabinoid system (ECS) is essential for controlling the homeostasis of the skin and that the ECS is modified in the presence of skin disease. It is plausible to expect that the lamellar junction of the hoof expresses cannabinoid receptors and that their expression could be affected by lamellar disease. The goal of this study was to characterise the cannabinoid receptor type 1 (CB1R) and type 2 (CB2R) and the G protein-coupled receptor 55 (GPR55) within the dermo-epidermal junction of the hooves of healthy and laminitic horses. The expression of the CB1R, CB2R, and GPR55 within the dermo-epidermal lamellar junction of six healthy and 12 laminitic hooves was studied using polymerase chain reaction (PCR) and immunofluorescence. Both the mRNA and protein expression of the CB1R, CB2R, and GPR55 were found in the dermo-epidermal lamellar junction of horse hooves. The immunolabelling was expressed by the epithelial cells of the primary and secondary laminae of healthy hooves (CB2R > GPR55 > CB1R). The presence of CB1R, CB2R, and GPR55 immunoreactivity in the healthy laminar epithelial cells, coupled with increased protein expression in pathological epithelial cells, provided strong motivation for future investigation. These findings suggest that cannabinoid compounds which interact with these receptors may influence lamellar healing and mitigate inflammation in hoof diseases, particularly laminitis.
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    language: English
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