Multi-omics analyses the effect of Bifidobacterium longum subsp. longum BL21 supplementation on overweight and obese subjects: a randomized, double-blind, placebo-controlled study.

Background: Bifidobacterium longum subsp. longum BL21 has demonstrated promise in alleviating gut microbiota disturbances and metabolic regulation in high-fat diet-induced obesity and type 2 diabetes mellitus models. However, the effect of probiotic B. longum BL21 on overweight and obese individuals...

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Publicado en:Nutrition & Metabolism Vol. 22; no. 1; pp. 1 - 12
Autores principales: Wang, Xiaoya, Xing, Zefeng, Wang, Rui, Zhang, Guoming, Liu, Guodong, Li, Zhen, Li, Lixiang
Formato: research tables/charts randomized controlled trial Journal Article
Publicado: BioMed Central 7/17/2025
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 7/17/2025
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      pub: BioMed Central
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        186711384
        10.1186/s12986-025-00969-2
        186711384
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        atl: Multi-omics analyses the effect of Bifidobacterium longum subsp. longum BL21 supplementation on overweight and obese subjects: a randomized, double-blind, placebo-controlled study.
      aug:
        au:
          Wang, Xiaoya
          Xing, Zefeng
          Wang, Rui
          Zhang, Guoming
          Liu, Guodong
          Li, Zhen
          Li, Lixiang
        affil: https://ror.org/056ef9489 Department of Gastroenterology, Qilu Hospital of Shandong University, 107 Wenhuaxi Road, 250012, Jinan, Shandong Province, China
      sug:
        subj:
          Obesity Diet Therapy
          Multiomics
          Probiotics Administration and Dosage
          Dietary Supplementation
          Gut Microbiota Prevention and Control
          Diabetes Mellitus, Type 2 Diet Therapy
          Dietary Fats Adverse Effects
          Treatment Outcomes
          Human
          Male
          Female
          Adult
          Middle Age
          Randomized Controlled Trials
          Random Assignment
          Comparative Studies
          Double-Blind Studies
          Body Weight
          Lipids
          Triglycerides Blood
          Biotin
          Descriptive Statistics
          Absorption
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Male
          Female
      ab: Background: Bifidobacterium longum subsp. longum BL21 has demonstrated promise in alleviating gut microbiota disturbances and metabolic regulation in high-fat diet-induced obesity and type 2 diabetes mellitus models. However, the effect of probiotic B. longum BL21 on overweight and obese individuals remain unclear. Methods: A randomized, double-blind, placebo-controlled trial was performed, and 66 adult individuals were assigned to receive either BL 21 (2*1010 colony-forming units per day along with 3 g of maltodextrin) or placebo (3 g of maltodextrin daily) for 8 weeks. Multi-omics analyses were employed to evaluate the impact of the B. longum strain BL21 on gut microbiota, serum metabolomics, body weight and lipids profiles in overweight and obese participants. Results: Following the intervention, both the BL21 group (1.22 ± 2.78, P = 0.02) and placebo group (0.98 ± 2.06, P = 0.01) demonstrated significant body weight reductions, with no statistically significant intergroup difference observed (P = 0.81). Notably, only the BL21 group exhibited a significant reduction in triglyceride levels compared to baseline (0.21 ± 1.09, P = 0.04). Microbiota analysis indicated that BL21 intervention significantly changed the β-diversity at week 8 compared with placebo group. The genera of Parasutterella, Parabacteroides, Blautia, Dorea, Butyricicoccus enriched in BL21 group. Metabolomics results indicated that sphingolipid metabolism, biotin metabolism and protein digestion and absorption were the top altered pathway in BL21 group compared with placebo group after intervention. Conclusion: B. longum subsp. longum BL21 may be a beneficial candidate to modulate the gut microbiota and triglyceride metabolism of overweight and obese individuals. Trial registration: Clinical trial registration number: NCT06140641. Date of registration: November 17, 2023.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        randomized controlled trial
        Journal Article
      ougenre: Article
    language: English
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