NETosis Genes and Pathomic Signature: A Novel Prognostic Marker for Ovarian Serous Cystadenocarcinoma.

To evaluate the prognostic significance and molecular mechanism of NETosis markers in ovarian serous cystadenocarcinoma (OSC), we constructed a machine learning-based pathomic model utilizing hematoxylin and eosin (H&E) slides. We analyzed 333 patients with OSC from The Cancer Genome Atlas for progn...

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Publicado en:Journal of Imaging Informatics in Medicine Vol. 38; no. 4; pp. 2412 - 2428
Autores principales: Zhan, Feng, Guo, Yina, He, Lidan
Formato: pictorial research tables/charts Journal Article
Publicado: Springer Nature Aug2025
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Aug2025
      vid: 38
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      pub: Springer Nature
      place: New York, New York
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        atl: NETosis Genes and Pathomic Signature: A Novel Prognostic Marker for Ovarian Serous Cystadenocarcinoma.
      aug:
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          Zhan, Feng
          Guo, Yina
          He, Lidan
        affil: https://ror.org/01cyb5v38 College of Engineering, Fujian Jiangxia University, Fuzhou, Fujian, China
      sug:
        subj:
          Ovarian Neoplasms Prognosis
          Neoplasms, Cystic, Mucinous, and Serous Prognosis
          Adenocarcinoma Prognosis
          Gene Expression
          Bioinformatics
          Prediction Models
          Machine Learning Algorithms
          Genetic Markers
          Human
          Female
          Middle Age
          Aged
          Aged, 80 and Over
          Funding Source
          Cancer Patients
          Support Vector Machine
          Logistic Regression
          Mutation
          Neoplasm Metastasis
          Neoplasm Invasiveness
          Overall Survival
          ROC Curve
          Kaplan-Meier Estimator
          Descriptive Statistics
          Disease Progression
          Genetic Screening
          Survival Analysis
          Cox Proportional Hazards Model
          Log-Rank Test
          Chi Square Test
          Spearman's Rank Correlation Coefficient
          Data Analysis Software
          Cluster Analysis
          Staining and Labeling
          Benzopyrans
          Fluorescent Dyes
          Middle Aged: 45-64 years
          Aged: 65+ years
          Aged, 80 & over
          Female
      ab: To evaluate the prognostic significance and molecular mechanism of NETosis markers in ovarian serous cystadenocarcinoma (OSC), we constructed a machine learning-based pathomic model utilizing hematoxylin and eosin (H&E) slides. We analyzed 333 patients with OSC from The Cancer Genome Atlas for prognostic-related neutrophil extracellular trap formation (NETosis) genes through bioinformatics analysis. Pathomic features were extracted from 54 cases with complete pathological images, genetic matrices, and clinical information. Two pathomic prognostic models were constructed using support vector machine (SVM) and logistic regression (LR) algorithms. Additionally, we established a predictive scoring system that integrated pathomic scores based on the NETcluster subtypes and clinical signature. We identified four NETosis genes significantly correlated with OSC prognosis, which were functionally associated with immune response, somatic mutations, tumor invasion, and metastasis. Five robust pathomic features were selected for overall survival prediction. The LR and SVM pathomic models demonstrated strong predictive performance for the NETcluster subtype classification through five-fold cross-validation. Time-dependent ROC analysis revealed excellent prognostic capability of the LR pathomic model's score for the overall survival (AUC values of 0.658, 0.761, and 0.735 at 36, 48, and 60 months, respectively), further validated by Kaplan–Meier analysis. The expression levels of NETosis genes greatly affected OSC patients' prognoses. The pathomic analysis of H&E slide pathological images provides an effective approach for predicting both NETcluster subtype and overall survival in OSC patients.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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