An integrated bioinformatics approach for identification of key modulators and biomarkers involved in atrial fibrillation.
Introduction: Atrial fibrillation (AFib) is a sustained form of cardiac arrhythmia that occurs due to sympathetic overdrive, neurohumoral and electrophysiological changes. Sympatho-renal modulatory approach via miRNA-based therapeutics is likely to be an important treatment option for AFib. The stud...
| Publicado en: | Journal of Cardiovascular & Thoracic Research Vol. 17; no. 2; pp. 109 - 121 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Tabriz University of Medical Sciences
Jun2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=187500718&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 187500718 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20085117 BL7R jtl: Journal of Cardiovascular & Thoracic Research issn: 20085117 maglogo: N pubinfo: dt: Jun2025 vid: 17 iid: 2 pid: 69573 pub: Tabriz University of Medical Sciences artinfo: ui: 187500718 187500718 187500718 10.34172/jcvtr.025.33347 187500718 ppf: 109 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: An integrated bioinformatics approach for identification of key modulators and biomarkers involved in atrial fibrillation. aug: au: Thahiem, Summan Ishtiaq, Ayesha Iftekhar, Faisal Jan, Muhammad Ishtiaq Murtaza, Iram affil: Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan sug: subj: Atrial Fibrillation Familial and Genetic Atrial Fibrillation Diagnosis MicroRNA Metabolism Biological Markers Metabolism Sympathetic Nervous System Metabolism Kidney Metabolism Bioinformatics Human Gene Expression Microarray Analysis Gene Expression Profiling Signal Transduction Receptors, Adrenergic, beta Calcium Receptors, G-Protein-Coupled Health Care Delivery, Integrated Molecular Structure Genetic Screening Health Screening Prospective Studies Early Diagnosis Genetics Metabolic Networks and Pathways Proteins Funding Source ab: Introduction: Atrial fibrillation (AFib) is a sustained form of cardiac arrhythmia that occurs due to sympathetic overdrive, neurohumoral and electrophysiological changes. Sympatho-renal modulatory approach via miRNA-based therapeutics is likely to be an important treatment option for AFib. The study was aimed to unravel the common miRNAs as therapeutic targets involved in sympatho- renovascular axis to combat AFib. Methods: We employed the bioinformatics approach to discover differentially expressed genes (DEGs) from microarray gene expression datasets GSE41177 and GSE79768 of AFib patients. Concomitantly, genes associated with sympathetic cardio-renal axis, from Genetic Testing Registry (GTR) of National Center for Biotechnology Information (NCBI) were also analyzed. Overlapping miRNAs that target the maximum number of genes across all three pathological conditions perpetuating AFib were shortlisted. To confirm the reliability of the identified miRNAs, differential expression analysis was performed on miRNA expression profiles GSE190898, GSE68475, GSE70887 and GSE28954 derived from AFib patient samples. Results: ShinyGO analysis revealed enrichment in beta-adrenergic signaling, calcium signaling, as well as G protein-coupled receptor (GPCR) signaling involved in post synaptic membrane potential. The intersection of top 10 modules in miRNA-mRNA network revealed hub miRNAs having highest node degree, maximum neighborhood component (MNC), and maximal clique centrality (MCC) scores. Differential expression analysis revealed hub miRNAs identified through integrated approach were found to be significantly dysregulated in AFib patients. Conclusion: This integrated approach identified 6 hub miRNAs, 4 reported (miR-101-3p, miR-23-3p, miR-27-3p, miR-25-3p) and 2 novel (miR-32-5p, miR-92-3p) miRNAs that might act as putative biomarkers for AFib. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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