Spatial Transcriptome Analysis Reveals Diverse Human Burn Wound Microenvironment.
Histologic analyses of burn tissue are unable to discern reversible injury. Advanced molecular profiling, such as bulk RNA‐sequencing, provides more detail; however, these methods lose spatial context. Spatial transcriptomics allows gene transcripts to be mapped to tissue locations, revealing the mo...
| Publicado en: | Wound Repair & Regeneration Vol. 33; no. 4; pp. 1 - 6 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jul/Aug2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=187573147&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 187573147 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jul/Aug2025 vid: 33 iid: 4 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 187573147 187573147 187573147 10.1111/wrr.70061 187573147 ppf: 1 ppct: 5 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Spatial Transcriptome Analysis Reveals Diverse Human Burn Wound Microenvironment. aug: au: Junak, Mary Khatri, Parth Zajac, Jocelyn Kubica, Phillip Yan, Di Dinh, Huy Q. Gibson, Angela L. F. affil: Department of Surgery, University of Wisconsin, Madison Wisconsin,, USA sug: subj: Burns Familial and Genetic Burns Microbiology Wounds and Injuries Familial and Genetic Wounds and Injuries Microbiology Gene Expression Profiling Evaluation Sequence Analysis Human Funding Source RNA Transcription Factors Gene Expression Skin Transplantation Biological Factors Signal Transduction Burn Patients ab: Histologic analyses of burn tissue are unable to discern reversible injury. Advanced molecular profiling, such as bulk RNA‐sequencing, provides more detail; however, these methods lose spatial context. Spatial transcriptomics allows gene transcripts to be mapped to tissue locations, revealing the molecular pathways activated in the burn tissue microenvironment, where the depth of injury guides prognosis. This work demonstrates the capability of spatial transcriptomics to detect spatial gene expression patterns in burn tissue. Specifically, we show that (i) spatially variable expressed genes are distinct across different burn depth regions, which would not be identified with bulk RNA‐sequencing, (ii) transcriptionally distinct burn tissue regions are defined by gene signatures associated with diverse cell types and biological pathways, and (iii) these spatial gene signatures are identified in a subset of previously published bulk samples, suggesting their potential application in large‐scale and integrated studies. Caveats of this technology in burn tissue are provided to guide future research. This study highlights the promise of spatial transcriptomics to understand the human burn wound microenvironment and identify specific regions with regenerative potential that can be the target of tailored therapeutics, providing an alternative to imprecise excision and skin grafting. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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