Vitamin B levels in older adults with pre-frailty and frailty: the impact of MTHFR and TCN2 polymorphisms and their association with global DNA methylation and physical performance.
Background: Frailty syndrome poses significant challenges in older populations. Understanding the genetic and biochemical factors associated with frailty is essential for effective management strategies. Methods: In this study, Thai older adults (≥ 60 years, n = 170) were assessed for physical param...
| Publicado en: | Nutrition & Metabolism Vol. 22; no. 1; pp. 1 - 15 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
BioMed Central
9/2/2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=187699407&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 187699407 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17437075 1CYX jtl: Nutrition & Metabolism issn: 17437075 maglogo: N pubinfo: dt: 9/2/2025 vid: 22 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 187699407 187699407 187699407 10.1186/s12986-025-01004-0 187699407 ppf: 1 ppct: 14 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Vitamin B levels in older adults with pre-frailty and frailty: the impact of MTHFR and TCN2 polymorphisms and their association with global DNA methylation and physical performance. aug: au: Chitta, Pitaksin Pratumvinit, Busadee Kaewboonruang, Witchayaporn Dawangpa, Atchara Khamrangsee, Saliltip Assantachai, Prasert Sutiwisesak, Rujapope Wongloet, Wongsathit Tencomnao, Tewin Sae-Lee, Chanachai affil: https://ror.org/01znkr924 Research Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand sug: subj: Frailty Syndrome Familial and Genetic Vitamin B Complex Blood Polymorphism, Genetic DNA Methylation Physical Performance Evaluation Frailty Syndrome Risk Factors Risk Assessment Biochemistry Community Living In Old Age Oxidoreductases Serum Globulins Human Male Female Aged Frailty Syndrome Therapy Vitamin B Deficiency Complications Thailand Colorimetry Exercise Test Walking Speed Grip Strength Folic Acid Genotype Correlational Studies Disease Attributes Genetic Variation Biological Markers Frailty Syndrome Diagnosis Epigenomics Funding Source Psychological Tests Vitamin B Complex Analysis Carrier Proteins Gene Expression Univariate Statistics Logistic Regression Alleles Odds Ratio Confidence Intervals Descriptive Statistics Spearman's Rank Correlation Coefficient Cross Sectional Studies Chi Square Test Fisher's Exact Test Mann-Whitney U Test One-Way Analysis of Variance Kruskal-Wallis Test Post Hoc Analysis Aged: 65+ years Male Female ab: Background: Frailty syndrome poses significant challenges in older populations. Understanding the genetic and biochemical factors associated with frailty is essential for effective management strategies. Methods: In this study, Thai older adults (≥ 60 years, n = 170) were assessed for physical parameters, levels of B vitamins, creatinine, and homocysteine. The polymorphisms of methylenetetrahydrofolate reductase (MTHFR) (677 C > T) and Transcobalamin II (TCN2) 776G > C were identified. Global DNA methylation (global DNAm) was assessed using a colorimetric assay. Results: Participants were categorised into robust (n = 61), pre-frail (n = 62), and frail (n = 47) groups by Fried criteria and Kihon checklist. The physical parameters, including chair stand, functional reach, gait speed, and handgrip strength, showed highly significant differences among the groups (p < 0.01). Significant differences in folate and vitamin B6 concentrations were observed between MTHFR and TCN2 genotypes, respectively. In addition, global DNAm levels were significantly lower in pre-frail individuals, particularly among those carrying the MTHFR C677T genotype, compared to both robust and frail groups. Notably, lower global DNAm was associated with a higher likelihood of being classified as pre-frail rather than frail, and a lower likelihood of being pre-frail compared to robust individuals. Moreover, correlation analyses revealed significant associations among physical parameters, clinical characteristics, and global DNAm. Conclusions: This study demonstrated the interplay between genetic variants, micronutrient status, and epigenetic modifications in the context of frailty among older adults. These findings highlight the potential of epigenetic and metabolic markers in identifying early frailty, though longitudinal and mechanistic studies are needed to further clarify causal pathways. Trial registration: This study was duly registered with the Thai Clinical Trial under the identifier TCTR20240626002 (date of registration: 21/06/2024). pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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