MyoAnalyst: an ImageJ plugin for accurate and automatic myofiber segmentation and analysis in skeletal muscle cross sections.
Quantifying myofiber size is essential for assessing the health and function of skeletal muscle. Although several ImageJ plugins are currently available for myofiber segmentation and size quantification, significant challenges remain—most notably limited accuracy and poor compatibility with hematoxy...
| Publicado en: | Histochemistry & Cell Biology Vol. 163; no. 1; pp. 1 - 14 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
9/5/2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=187782778&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 187782778 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: 9/5/2025 vid: 163 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 187782778 10.1007/s00418-025-02414-0 187782778 ppf: 1 ppct: 13 formats: tig: atl: MyoAnalyst: an ImageJ plugin for accurate and automatic myofiber segmentation and analysis in skeletal muscle cross sections. aug: au: Zhang, Bao Wang, Shuaiyu Wang, Yaning Liang, Chen Zhang, Hongbo affil: https://ror.org/018jdfk45 Medical College of Jiaying University, 514031, Meizhou, China sug: ab: Quantifying myofiber size is essential for assessing the health and function of skeletal muscle. Although several ImageJ plugins are currently available for myofiber segmentation and size quantification, significant challenges remain—most notably limited accuracy and poor compatibility with hematoxylin and eosin (H&E)-stained skeletal muscle cross sections. In this study, we introduce MyoAnalyst, an ImageJ plugin designed to enable automated analysis of both immunofluorescence (IF)- and H&E-stained skeletal muscle cross sections. Compared to existing ImageJ plugins, MyoAnalyst delivers enhanced segmentation sensitivity and superior boundary delineation accuracy across both healthy and injured muscle tissue stained with IF. Importantly, it also supports fully automated analysis of H&E-stained sections. With its intuitive graphical interface and batch processing capabilities, MyoAnalyst provides a potentially efficient tool for myofiber size quantification in both research and clinical settings. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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