Helicobacter pylori real-time quantitative PCR to examine efficacy of endoscope processing.
Helicobacter pylori (H. pylori) is the main cause of peptic ulcer disease. The primary aim of this research is to determine the effectiveness of current endoscope High-Level Disinfection (HLD) at clearing H. pylori. The secondary aim is to evaluate the prevalence of H. pylori in patients undergoing...
| Publicado en: | American Journal of Infection Control Vol. 53; no. 10; pp. 1076 - 1080 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Oct2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=187941853&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 187941853 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01966553 44Q jtl: American Journal of Infection Control issn: 01966553 maglogo: N pubinfo: dt: Oct2025 vid: 53 iid: 10 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 187941853 187941853 187941853 10.1016/j.ajic.2025.07.001 187941853 ppf: 1076 ppct: 4 formats: tig: atl: Helicobacter pylori real-time quantitative PCR to examine efficacy of endoscope processing. aug: au: Mussina, L. Khoury, F. Araujo, D. Yadlapalli, M. Pezzone, M. Yassin, M. affil: Infectious Diseases and Microbiology, School of Public Health University of Pittsburgh, Pittsburgh, PA sug: subj: Sterilization and Disinfection Evaluation Endoscopes Helicobacter Pylori Equipment Contamination Prevention and Control Helicobacter Infections Epidemiology Reverse Transcriptase Polymerase Chain Reaction Endoscopy, Gastrointestinal Human Prospective Studies Record Review Microbiological Techniques Urease Analysis Biopsy Gastric Lavage DNA Sensitivity and Specificity Descriptive Statistics ab: Helicobacter pylori (H. pylori) is the main cause of peptic ulcer disease. The primary aim of this research is to determine the effectiveness of current endoscope High-Level Disinfection (HLD) at clearing H. pylori. The secondary aim is to evaluate the prevalence of H. pylori in patients undergoing esophagogastric-duodenoscopy (EGD). This is a prospective study collecting samples from esophagogastroduodenoscopy (EGD) for H. pylori. testing via gastric lavage and after HLD via flushing endoscope with sterile water. The patients' records were reviewed and the fluid obtained was tested for microbiologic culture; urease testing, and qPCR testing using UreA primers and probe. The study included 202 samples (101 patients). H. pylori was positive in 37%, 21.9% and 2.4% of samples using Urease testing, culture and biopsy respectively. H. pylori was four times more likely to be identified via gastric lavage than by biopsy. qPCR was significantly more likely to be negative after HLD (27 vs 3 patients). HLD was effective in reducing H. pylori but was not able to totally eliminate H. pylori DNA. qPCR is more sensitive than routine culture but can't accurately determine potential for infection transmission. Gastric lavage may be more effective in detecting H. pylori than histology. • qPCR is an important tool to assess HLD effectiveness. • HLD reduces H pylori DNA in endoscopes. • H pylori prevalence may be lower than expected. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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