Bacterial profile and biofilm intensity on endotracheal tubes following chlorhexidine use in critically ill patients.
Objective: To identify bacterial species colonizing the endotracheal tube (ETT) and to evaluate the extent of biofilm formation in patients undergoing oral hygiene protocols with 0.2% chlorhexidine. In addition, the study aimed to determine the correlation between the duration of ETT placement and t...
| Publicado en: | Critical Care & Shock Vol. 28; no. 5; pp. 207 - 215 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Critical Care & Shock Journal
2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=188342749&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 188342749 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14107767 6CKO jtl: Critical Care & Shock issn: 14107767 maglogo: N pubinfo: dt: 2025 vid: 28 iid: 5 pid: 45459 pub: Critical Care & Shock Journal place: Houston, Texas artinfo: ui: 188342749 188342749 188342749 188342749 ppf: 207 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Bacterial profile and biofilm intensity on endotracheal tubes following chlorhexidine use in critically ill patients. aug: au: Rizkiya, Putri Semedi, Bambang Pujo Kusuma, Edward Widodo, Agung Dwi Wahyu affil: Department of Anesthesiology and Reanimation, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia sug: subj: Bacterial Colonization Endotracheal Tubes Biofilms Chlorhexidine Therapeutic Use Oral Hygiene Critically Ill Patients Human Indonesia Female Male Nonexperimental Studies Analytic Research Intensive Care Units Microbiological Techniques Enzyme-Linked Immunosorbent Assay Culture Media Staining and Labeling Gram-Negative Bacteria Klebsiella Pseudomonas Acinetobacter Infections Staphylococcus Fisher's Exact Test Data Analysis Software Descriptive Statistics Pneumonia, Ventilator-Associated Female Male ab: Objective: To identify bacterial species colonizing the endotracheal tube (ETT) and to evaluate the extent of biofilm formation in patients undergoing oral hygiene protocols with 0.2% chlorhexidine. In addition, the study aimed to determine the correlation between the duration of ETT placement and the degree of bacterial biofilm formation. Design: Observational analytic study. Setting: Intensive Care Unit (ICU) of Dr. Soetomo General Hospital, Surabaya, Indonesia. Patients and participants: Endotracheal tubes were collected from 41 mechanically ventilated ICU patients who had undergone routine oral hygiene with 0.2% chlorhexidine. Patients were selected consecutively, and all ETTs were processed for microbiological analysis with the roll-plate culture method and the enzyme-linked immunosorbent assay (ELISA) biofilm microplate reader. Interventions: There was no additional therapeutic intervention beyond standard ICU care, including ventilator-associated pneumonia (VAP) bundles and chlorhexidine-based oral hygiene every 8 hours. Measurements and results: A total of 67 bacterial isolates were obtained from the oral-exposed surface of ETTs. Among these, 85% (59 isolates) demonstrated biofilm-forming capability, with Klebsiella pneumoniae (33%), Pseudomonas aeruginosa (21%), and Acinetobacter baumannii (10%) being the most common species. Gram-negative bacteria formed the majority of biofilm-positive isolates and exhibited stronger biofilm intensity compared to Gram-positive bacteria. No statistically significant correlation was observed between ETT usage duration and biofilm intensity (p>0.05). Conclusions: High rates of biofilm formation were identified on the outer surfaces of ETTs in ICU patients despite routine chlorhexidine oral care. No significant association was observed between the duration of ETT use and biofilm intensity. These findings highlight the need for additional preventive strategies beyond chlorhexidine to reduce the risk of biofilm-associated complications in mechanically ventilated patients. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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