Evaluation of Citral and Green Silver Nanoparticles From Cymbopogon citratus Extract on Biochemical Profile and Nrf2 Gene Expression in Liver Tissue of Type 2 Diabetic Rats.

The aim of this study was to evaluate the antioxidant effects of Cymbopogon citratus, citral, and green‐synthesized silver nanoparticles derived from the extract in mitigating oxidative stress–induced liver damage in diabetes mellitus. Seventy rats were randomly split into seven groups: a control gr...

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Published in:BioMed Research International Vol. 2025; pp. 1 - 11
Main Authors: Khatinasab, Shadi, Kazemipour, Nasrin, Noorbakhsh, Mohammad Foad, Nazifi, Saeed, Faraji, Milad, Ahmadi, Nasrollah, Badraoui, Riadh
Format: pictorial research tables/charts Journal Article
Published: Wiley-Blackwell 10/14/2025
Online Access:View this record in EBSCOhost
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      dt: 10/14/2025
      vid: 2025
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        188672144
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        188672144
        10.1155/bmri/9266092
        188672144
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        atl: Evaluation of Citral and Green Silver Nanoparticles From Cymbopogon citratus Extract on Biochemical Profile and Nrf2 Gene Expression in Liver Tissue of Type 2 Diabetic Rats.
      aug:
        au:
          Khatinasab, Shadi
          Kazemipour, Nasrin
          Noorbakhsh, Mohammad Foad
          Nazifi, Saeed
          Faraji, Milad
          Ahmadi, Nasrollah
          Badraoui, Riadh
        affil: Department of Basic Sciences,, School of Veterinary Medicine,, Shiraz University,, Shiraz, Iran, shirazu.ac.ir
      sug:
        subj:
          Plant Extracts Pharmacodynamics
          Lemon Grass Pharmacodynamics
          Nanoparticles
          Terpenes Pharmacodynamics
          Antioxidants
          NF-E2-Related Factor 2 Metabolism
          Gene Expression Drug Effects
          Liver Drug Effects
          Diabetes Mellitus, Type 2 Drug Therapy
          Oxidative Stress Drug Effects
          Silver
          Animal Studies
          Rats
          Olive Oil
          Metformin
          Liver Function Tests
          Aspartate Aminotransferase
          Alkaline Phosphatase
          Blood Glucose
          Metformin Administration and Dosage
          Alanine Aminotransferase
          Reverse Transcriptase Polymerase Chain Reaction
          Analysis of Variance
          Post Hoc Analysis
          Funding Source
      ab: The aim of this study was to evaluate the antioxidant effects of Cymbopogon citratus, citral, and green‐synthesized silver nanoparticles derived from the extract in mitigating oxidative stress–induced liver damage in diabetes mellitus. Seventy rats were randomly split into seven groups: a control group of healthy rats received normal saline, a cosolvent group of Type 2 diabetics received olive oil, a negative control group of Type 2 diabetics, and groups of diabetic rats receiving various treatments: metformin (100 mg/kg), Cymbopogon citratus extract (30 mg/kg), citral (30 mg/kg), and green‐synthesized silver nanoparticles (30 mg/kg). Type 2 diabetes was induced in rats using 90 mg/kg of nicotinamide and 65 mg/kg of streptozotocin. After 6 weeks, the rats were anesthetized to extract blood samples. The serum levels of the liver function test (serum glutamic–oxaloacetic transaminase, serum glutamic–pyruvic transaminase, and alkaline phosphatase) were measured. They were then euthanized by CO2 inhalation. Liver tissue was used to investigate histopathology, antioxidant activity, and Nrf2 gene expression. In the diabetes group, liver function tests increased, while TAC decreased and MDA increased (p < 0.05). The expression of the Nrf2 gene decreased compared to the control group (p < 0.05). In the treatment groups, the liver function tests and MDA decreased, while TAC and the expression of the Nrf2 gene increased compared to the Type 2 diabetes group (p < 0.05). The histopathological examination showed a return to normal condition. The study found that Cymbopogon citratus, specifically citral and green‐synthesized silver nanoparticles, effectively mitigated oxidative stress–induced liver damage in diabetes.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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