Potential of Heparan Sulphate Mimetics Integrated Into Collagen Scaffolds for Enhanced Skin Wound Healing.
Optimal healing of full‐thickness skin wounds remains a clinical challenge. While current skin substitutes aid burn wound management, there is still a need to effectively minimize scarring. Therefore, we developed type I collagen scaffolds with covalently bound ReGeneraTing Agent (RGTA) OTR4120 (OTR...
| Publicado en: | Wound Repair & Regeneration Vol. 33; no. 5; pp. 1 - 14 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Sep/Oct2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=188875053&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 188875053 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Sep/Oct2025 vid: 33 iid: 5 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 188875053 188875053 188875053 10.1111/wrr.70096 188875053 ppf: 1 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Potential of Heparan Sulphate Mimetics Integrated Into Collagen Scaffolds for Enhanced Skin Wound Healing. aug: au: Avila‐Martinez, Nancy Krymchenko, Roman Gansevoort, Merel Pfirrmann, Maren Aparicio, Sofia Choppin, Agnes Chiappini, Franck Barritault, Denis Verdoes, Martijn van Kuppevelt, Toin H. Boekema, Bouke K. H. L. Daamen, Willeke F. affil: Radboud university medical center, Research Institute for Medical Innovation, Department of Medical BioSciences, Nijmegen, the Netherlands sug: subj: Glycosaminoglycans Collagen Tissue Scaffolds Wound Healing Skin Care Macrophages Metabolism Models, Biological Animal Studies Rats In Vivo Studies Human In Vitro Studies Wound Care Histocytochemistry Blotting, Western Fluorescent Antibody Technique Microscopy, Electron, Scanning Monocytes Metabolism Flow Cytometry Biological Markers Blood Enzyme-Linked Immunosorbent Assay Gene Expression Cytokines Analysis Interleukins Analysis Wound Measurement Descriptive Statistics One-Way Analysis of Variance Two-Way Analysis of Variance Post Hoc Analysis Data Analysis Software Funding Source ab: Optimal healing of full‐thickness skin wounds remains a clinical challenge. While current skin substitutes aid burn wound management, there is still a need to effectively minimize scarring. Therefore, we developed type I collagen scaffolds with covalently bound ReGeneraTing Agent (RGTA) OTR4120 (OTR), a synthetic heparan sulphate analogue resistant to glycanase degradation (Col I + OTR). To further stimulate skin regeneration, collagen scaffolds with and without OTR4120 were subsequently loaded with sonic hedgehog (SHH), a key effector molecule in embryogenesis. The presence of OTR4120 and SHH in scaffolds was biochemically and histologically confirmed after crosslinking and sterilization. SHH was found deeper into collagen scaffolds in the presence of OTR4120. Addition of SHH to scaffolds showed lower expression of M1‐like cell surface markers, while Col I + OTR significantly enhanced IL‐10 production. The potential of OTR4120 in wound healing was further evaluated in vivo using a rat full‐thickness wound model over 28 days. By day 14, macroscopic images revealed that OTR‐treated wounds better maintained the original wound shape. Histological analysis showed increased blood vessel formation, fewer scaffold remnants and more contiguous sebaceous glands in the granulation tissue with Col I + OTR scaffolds. This study demonstrates that OTR4120 could be a promising addition to acellular skin substitutes for improving acute wound healing. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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