Detection of aggR and agg4A Genes and Antibiotic Resistance Profiles in Biofilm-Forming Escherichia coli Clinical Isolates from Miandoab Hospitals.
Introduction: Escherichia coli (E.coli) is a non-pathogenic facultative bacterium that is the predominant pathogen in the human intestine, however, some of its strains are known to be the cause of many human infections. The major adhesins of enteroaggregative strains has aggR gene encodes AAF-I. Thi...
| Published in: | International Journal of Travel Medicine & Global Health Vol. 13; no. 3; pp. 135 - 142 |
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| Main Authors: | , , |
| Format: | pictorial research tables/charts Journal Article |
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Tarbiat Modares University Press
Sep2025
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=189099496&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 189099496 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23221100 FUA0 jtl: International Journal of Travel Medicine & Global Health issn: 23221100 maglogo: N pubinfo: dt: Sep2025 vid: 13 iid: 3 pid: 93586 pub: Tarbiat Modares University Press artinfo: ui: 189099496 189099496 189099496 10.30491/ijtmgh.2025.519504.1474 189099496 ppf: 135 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Detection of aggR and agg4A Genes and Antibiotic Resistance Profiles in Biofilm-Forming Escherichia coli Clinical Isolates from Miandoab Hospitals. aug: au: Bagheri, Marjan bonab, Zahra Hojjati Soltanzadeh, Hossein affil: Department of Microbiology, Bon.C., Islamic Azad University, Bonab, Iran sug: subj: Genes Drug Resistance, Microbial Biofilms Escherichia Coli Microbiological Techniques Hospitals Iran Urinary Tract Infections Microbiology Human Urinalysis Gram-Negative Bacteria Inpatients Outpatients Staining and Labeling Iran Culture Media Descriptive Statistics Data Analysis Software T-Tests One-Way Analysis of Variance ab: Introduction: Escherichia coli (E.coli) is a non-pathogenic facultative bacterium that is the predominant pathogen in the human intestine, however, some of its strains are known to be the cause of many human infections. The major adhesins of enteroaggregative strains has aggR gene encodes AAF-I. This strain has too agg4A encodes AAF-IV, each of which has a specific role. Various factors, such as the high volume of antibiotic use, poor hygiene conditions contribute to the development and spread of antibiotic resistance. Methods: Urine samples were taken from 100 patients referred to Miandoab Hospital in Iran and cultured in Eosin methylene blue(EMB) selective culture medium. E. coli differential test was performed for positive samples. Antibiogram test was used to investigate antibiotic resistance. PCR was performed for investigation the presence of aggR and agg4A genes. Results: In this study, 80% of the samples were resistant to the antibiotic ampicillin and 20% were sensitive to the antibiotic ampicillin. In this study, 20% of the samples formed weak biofilm, 20% of the samples formed medium biofilm, 50% of the samples formed strong biofilm, and 10% of the samples formed very strong biofilm. One sample (5%) had the aggR gene and six samples (30%) had the agg4A gene. Conclusion: According to the results of this study, aggR and agg4A, as genes effective in biofilm biosynthesis, have different effects on biofilm formation of clinical isolates of E.coli by PCR method, and these effects are exerted through the influence on the expression of genes involved in biofilm formation. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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