Overexpression of KDM6A in Hepatoma Cells Induces Hepatocytic Differentiation and Attenuates Proliferation Rate, Colony Formation, and Migration Capacities.
Objective: Despite the remarkable advances in approved therapeutic approaches, the recurrence rate of hepatocellular carcinoma (HCC) is very high after treatment. Therefore, introducing innovative therapeutic modalities such as targeted molecular therapies is inevitable. Lysine demethylase 6A (KDM6A...
| Publicado en: | BioMed Research International Vol. 2025; pp. 1 - 16 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
11/15/2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=189328359&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 189328359 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 11/15/2025 vid: 2025 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 189328359 189328359 189328359 10.1155/bmri/5551687 189328359 ppf: 1 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Overexpression of KDM6A in Hepatoma Cells Induces Hepatocytic Differentiation and Attenuates Proliferation Rate, Colony Formation, and Migration Capacities. aug: au: Hashemi, Mahdieh Hesaraki, Mahdi Ramezankhani, Roya Yaghoubi, Seyyed Mohammad Shokouhian, Bahare Piryaei, Abbas Rismani, Elham Najimi, Mustapha Vosough, Massoud Miceli, Vitale affil: Department of Regenerative Medicine,, Cell Science Research Center,, Royan Institute for Stem Cell Biology and Technology,, ACECR,, Tehran, Iran, royaninstitute.org sug: subj: Carcinoma, Hepatocellular Cell Line, Tumor Gene Expression Cell Proliferation Genetic Variation Cell Differentiation Colony-Forming Units Assay Cell Movement Human Funding Source Descriptive Statistics Data Analysis Software Analysis of Variance T-Tests Enzyme-Linked Immunosorbent Assay Blotting, Western Biological Assay ab: Objective: Despite the remarkable advances in approved therapeutic approaches, the recurrence rate of hepatocellular carcinoma (HCC) is very high after treatment. Therefore, introducing innovative therapeutic modalities such as targeted molecular therapies is inevitable. Lysine demethylase 6A (KDM6A) is a member of the KDM6 family with histone demethylase activity. This gene frequently mutates in different cancers, and its mutations are associated with the increased likelihood of carcinogenesis. This study is aimed at evaluating if inducing KDM6A expression could attenuate cancerous features of HCC cells. Method: A lentiviral‐based vector was used to induce KDM6A expression in Huh‐7 cells. The impact of KDM6A overexpression on the cancerous phenotype of HCC cells was assessed by measuring proliferation rate, migration and colony formation capacity, and differentiation induction toward hepatocytes. Results: KDM6A overexpression significantly altered cellular morphology, proliferation rate, cell cycle pattern, colony formation, and migration capacity of HCC cells. In addition, induction of differentiation toward hepatocytic fate resulted in down/upregulation of epithelial–mesenchymal transition (EMT) markers associated with the cadherin switch. Furthermore, the expressions of ALB and HNF4α, key hepatocytic hallmarks, were increased. Conclusion: Overexpression of KDM6A could be used as a potential noninvasive molecular therapeutic strategy to prevent metastasis and recurrence rate in HCC. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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