Radiopharmaceutical kit to prepare Copper-64 labelled porphyrin-lipid nanoparticles for radiotracer imaging studies in cancer patients.
Purpose: PORPHYSOMES (PS) are multifunctional porphyrin-lipid nanoparticles for fluorescence-guided photochemical tumour ablation and immune stimulation. PS can be radiolabelled with Copper-64 (64Cu-PS) to permit tracing of their whole-body distribution and uptake in tumours with nuclear imaging. He...
| Publicado en: | European Journal of Nuclear Medicine & Molecular Imaging Vol. 53; no. 1; pp. 663 - 687 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Dec2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=189634280&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 189634280 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16197070 NPC jtl: European Journal of Nuclear Medicine & Molecular Imaging issn: 16197070 maglogo: N pubinfo: dt: Dec2025 vid: 53 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 189634280 186695427 10.1007/s00259-025-07442-5 189634280 ppf: 663 ppct: 24 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Radiopharmaceutical kit to prepare Copper-64 labelled porphyrin-lipid nanoparticles for radiotracer imaging studies in cancer patients. aug: au: Valic, Michael S. Fisher, Carl J. Zheng, Mark Gregor, Alexander M. Jiang, Wenlei Chan, Conrad Chan, Harley H. L. El-Sayes, Abdullah Zhang, Chris J. Ye, Tina Halim, Michael Schimmer, Pamela Li, Celina Bernards, Nicholas Chu, Arthur C. K. Cai, Zhongli Chen, Juan Irish, Jonathan C. Yasufuku, Kazuhiro Weersink, Robert affil: https://ror.org/042xt5161 Princess Margaret Cancer Centre, University Health Network, 101 College Street, PMCRT RM 5-354, M5G 1L7, Toronto, ON, Canada sug: ab: Purpose: PORPHYSOMES (PS) are multifunctional porphyrin-lipid nanoparticles for fluorescence-guided photochemical tumour ablation and immune stimulation. PS can be radiolabelled with Copper-64 (64Cu-PS) to permit tracing of their whole-body distribution and uptake in tumours with nuclear imaging. Herein we characterised the physicochemical and radiochemical properties of PS and 64Cu-PS and evaluated their pharmacology and toxicology in rats and dogs. Methods: 64Cu-PS labelling procedure was optimised and developed into a "one pot" kit format. The plasma pharmacokinetics (PK), tissue distribution, and single and repeat-dose toxicity of dose escalating PS was evaluated after intravenous injection in healthy Fisher 344 rats and Beagle dogs. Radiation dosimetry from single 64Cu-PS dose was estimated in humans. Results: Kit prepared 64Cu-PS had high labelling yields and mean specific activities of 18.5 MBq/mg (11.4 GBq/mg max). The plasma PK of PS was linear between doses 0.5–15 mg/kg in both rats and dogs. Tissue distribution was primarily to the liver. The estimated effective dose from 64Cu-PS in humans was 38–47 µSv/MBq. Single doses of 32.6 mg/kg PS did not have detectable toxicities in rats. In dogs, mild transient infusion reactions were observed following single and repeat-doses of PS starting at ≥ 10 mg/kg PS. Doses up to 15 mg/kg PS were tolerated in dogs. Conclusions: The pharmacology and safety profiles of PS and 64Cu-PS in rats and dogs did not raise concerns regarding their use in humans. Radiolabelled 64Cu-PS will permit accurate imaging-based assessments of nanoparticle PK and tumour uptake in imaging studies in cancer patients. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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