Evaluation of an internet of things device for isothermal molecular detection.

We describe a low-cost up scalable molecular test platform providing accessibility to frequent testing - the prerequisite to control infectious epidemics. The device uses the Multi-Spectral Digital Sensor AS7341 in a small fluorescence spectrometer which communicates with a Smartphone App via Blueto...

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Publicado en:Infection Vol. 53; no. 6; pp. 2467 - 2481
Autores principales: Weidmann, Manfred, Alvarez, Jesus Bueno, Zinn, Steffen, Ibragimov, Ruslan, Hasani, Mohammed, Graf, Elena, Weber, Aiko, Arrey, Frida, Szczygiel, Marcelina Marta, Kowalewska, Zuzanna, Kobialka, Rea Maja, Ceruti, Arianna, Wahed, Ahmed Abd El, Diebold, Michael, Wahnes, Christian
Formato: pictorial research tables/charts Journal Article
Publicado: Springer Nature Dec2025
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Dec2025
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s15010-025-02581-1
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          Weidmann, Manfred
          Alvarez, Jesus Bueno
          Zinn, Steffen
          Ibragimov, Ruslan
          Hasani, Mohammed
          Graf, Elena
          Weber, Aiko
          Arrey, Frida
          Szczygiel, Marcelina Marta
          Kowalewska, Zuzanna
          Kobialka, Rea Maja
          Ceruti, Arianna
          Wahed, Ahmed Abd El
          Diebold, Michael
          Wahnes, Christian
        affil: Midge Medical GmbH, Berlin, Germany
      sug:
        subj:
          COVID-19 Testing
          Point-of-Care Testing
          Molecular Diagnostic Techniques
          Internet of Things Evaluation
          Human
          Descriptive Statistics
          Data Analysis Software
          Confidence Intervals
          Algorithms
          Reverse Transcriptase Polymerase Chain Reaction
          RNA
          Sensitivity and Specificity
      ab: We describe a low-cost up scalable molecular test platform providing accessibility to frequent testing - the prerequisite to control infectious epidemics. The device uses the Multi-Spectral Digital Sensor AS7341 in a small fluorescence spectrometer which communicates with a Smartphone App via Bluetooth, which passes on the raw data to outsourced computation in the cloud via the internet. This very stable setup allows easy to use on site testing of individual samples and anonymised data analysis while providing the basis for direct result transmission of a rapid isothermal molecular diagnostic test result not only to the user but also to public health bodies. This internet of things (IoT) platform allows to collect real molecular test data from users with usability and industrial up scalability at the heart of the design. The device characteristics are described in detail and the performance for a rapid SARS-CoV-2 Recombinase Polymerase Amplification assay achieved 98.6% sensitivity and 98% specificity testing 148 positive and 501 negative samples.
      pubtype: Academic Journal
      doctype:
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        research
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      ougenre: Article
    language: English
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