Phenolic Compounds of Justicia gendarussa Show Pharmacological Potentials Against Pain, Oxidation, Hyperglycemia, Diarrhea, and Microbes: Phytopharmacological and Computational Approaches.

Background: Justicia gendarussa is a branched shrub spread across Indian, Sri Lankan, and Malaysian forests. It has been widely used across many countries to treat asthma, rheumatism, colics in children, eczema, and HIV. The study goal was to investigate the phytoconstituents from J. gendarussa and...

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Publicado en:BioMed Research International Vol. 2025; pp. 1 - 21
Autores principales: Munny, Farzana Akter, Islam, Mehedi, Akter, Mahafuza, Ferdousi, Mushtahsin, Asif, Md. Moaz Ahmed, Hossain, Md. Solaiman, Sharmin, Sabrina, Zahidul Islam, Md, Hossain, Md. Aslam, Islam, Md. Rabiul, Banerjee, Baisakhi
Formato: equations & formulas pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 12/9/2025
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 12/9/2025
      vid: 2025
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        189914071
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        10.1155/bmri/2561508
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        atl: Phenolic Compounds of Justicia gendarussa Show Pharmacological Potentials Against Pain, Oxidation, Hyperglycemia, Diarrhea, and Microbes: Phytopharmacological and Computational Approaches.
      aug:
        au:
          Munny, Farzana Akter
          Islam, Mehedi
          Akter, Mahafuza
          Ferdousi, Mushtahsin
          Asif, Md. Moaz Ahmed
          Hossain, Md. Solaiman
          Sharmin, Sabrina
          Zahidul Islam, Md
          Hossain, Md. Aslam
          Islam, Md. Rabiul
          Banerjee, Baisakhi
        affil: Department of Pharmaceutical Chemistry,, Faculty of Pharmacy,, University of Dhaka,, Dhaka, Bangladesh, du.ac.bd
      sug:
        subj:
          Phenols Analysis
          Phenols Pharmacodynamics
          Pain Drug Therapy
          Oxidative Stress Drug Effects
          Hyperglycemia Drug Therapy
          Diarrhea Drug Therapy
          Microbial Contamination Prevention and Control
          Plants, Medicinal Therapeutic Use
          Plants, Medicinal Pharmacodynamics
          Plants, Medicinal Analysis
          Antioxidants Pharmacodynamics
          Antilipemic Agents Pharmacodynamics
          Antidiarrheals Pharmacodynamics
          Antiinfective Agents Pharmacodynamics
          Analgesics Pharmacodynamics
          Drug Efficacy Evaluation
          Plant Extracts Therapeutic Use
          Plant Extracts Pharmacodynamics
          Plant Extracts Analysis
          Animal Studies
          Molecular Structure
          Microbial Contamination
          Phytochemicals Therapeutic Use
          Phytochemicals Pharmacodynamics
          Bioinformatics
          Molecular Docking Simulation
          Mice
          Models, Biological
          Chromatography, Liquid
          Terpenes Pharmacodynamics
          Phytosterols Pharmacodynamics
          Glycerides Pharmacodynamics
          Magnetic Resonance Spectroscopy
          Free Radical Scavengers Drug Effects
          Antioxidants Administration and Dosage
          Antilipemic Agents Administration and Dosage
          Antidiarrheals Administration and Dosage
          Antiinfective Agents Administration and Dosage
          Analgesics Administration and Dosage
          Glutathione Drug Effects
          Receptors, Cell Surface Drug Effects
          Membrane Transport Proteins Drug Effects
          Cox-2 Inhibitors Drug Effects
          Oxidoreductases Drug Effects
          Plant Leaves Analysis
          Plant Leaves Pharmacodynamics
          In Vivo Studies
          In Vitro Studies
          Microbial Culture and Sensitivity Tests
          Dose-Response Relationship, Drug
          Computer Simulation
          Liquid Chromatography-Mass Spectrometry
          Hypoglycemic Agents Pharmacodynamics
          Gram-Positive Bacteria Drug Effects
          Gram-Negative Bacteria Drug Effects
          Fungi Drug Effects
          Glyburide Analysis
          Receptors, Opioid Drug Effects
          Descriptive Statistics
          Data Analysis Software
          Analysis of Variance
      ab: Background: Justicia gendarussa is a branched shrub spread across Indian, Sri Lankan, and Malaysian forests. It has been widely used across many countries to treat asthma, rheumatism, colics in children, eczema, and HIV. The study goal was to investigate the phytoconstituents from J. gendarussa and to discover its therapeutic potential against various disease conditions. Methods: The plant sample was collected, dried, and grinded into coarse powder which was then soaked in methanol for 2 weeks. After the maceration process, the crude methanolic extract was subjected to solvent–solvent partitioning into four different fractions: n‐hexane soluble fraction (HSF), dichloromethane soluble fraction (DMSF), ethyl acetate soluble fraction (EASF), and aqueous fraction (AQF). DMSF was chemically evaluated through chromatographic separation, and all the fractions including the crude methanolic extracts were assessed for their potential pharmacological activities against pain, oxidative stress, hyperglycemia, diarrhea, and microbes following standard protocols. Results: Chemical investigation results in the isolation of lupeol, β‐sitosterol, and 1‐monostearin. The structures of the compounds were elucidated through meticulous NMR spectroscopic analysis. In a DPPH free radical scavenging assay, prominent action was noticed by EASF, with a median inhibition concentration (IC50) of 24.207 g/mL in comparison to the BHT with an IC50 value of 23.159 g/mL. In central analgesic activity, all the results were highly significant, with the highest (233.47%) time elongation in comparison to the control, observed after 90 min at 600 mg/kg b.w. and maximum peripheral analgesic activity of 61.96% was found at a dose of 600 mg/kg b.w. Two test doses (600 and 400 mg/kg b.w.) demonstrated substantial hypoglycemic and antidiarrheal effects that became more pronounced over time. The isolated compounds demonstrated impressive binding scores when interacting with glutathione reductase (3GRS), mu‐opioid receptor (MOR), kappa opioid receptor (KOR), and glucose transporter 3 (GLUT 3) receptors. However, their performance was notably lacking in terms of binding with cyclooxygenase‐2 (COX‐2) and dihydrofolate reductase (DHFR) receptors. Conclusion: Three isolated phytochemicals demonstrate promising binding affinities with the receptor molecules that support the pharmacological findings of this study. However, additional research needs to be conducted to isolate more phytoconstituents and affirm the pharmacological potential of J. gendarussa.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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