Molecular epidemiology of bacterial enteric pathogens associated with diarrhea in children under 5 years of age in Lagos State, Nigeria.
Infectious diarrhea remains a widespread, contagious illness, posing severe challenges in developing countries. The aim of the study is to determine the molecular epidemiology of bacterial enteric pathogens associated with diarrhea in children under five years in Lagos State. A total of 268 particip...
| Publicado en: | Australian Journal of Medical Science Vol. 46; no. 4; pp. 165 - 178 |
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| Autores principales: | , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Australian Institute of Medical Scientists
Nov2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=190460202&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 190460202 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10381643 GD50 jtl: Australian Journal of Medical Science issn: 10381643 maglogo: N pubinfo: dt: Nov2025 vid: 46 iid: 4 pid: 88256 pub: Australian Institute of Medical Scientists place: Milton, Queensland, <Blank> artinfo: ui: 190460202 190460202 190460202 190460202 ppf: 165 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Molecular epidemiology of bacterial enteric pathogens associated with diarrhea in children under 5 years of age in Lagos State, Nigeria. aug: au: Ezioma, Dike Adobi Omosigho, Pius Omoruyi Sulaiman, Giwa Bridget, Omene Ogheneakporobo Eze, Glory Obiageli Omoruyi, Ewean chukwuma Okesanya, Olalekan John affil: Department of Medical Laboratory Science, Faculty of Applied Health Sciences, Edo State University, Iyamho, Edo State, Nigeria sug: subj: Diarrhea Epidemiology Epidemiology, Molecular Nigeria Diarrhea Risk Factors Microbiological Techniques Child Health Risk Assessment Diarrhea Microbiology Nigeria Human Male Female Infant, Newborn Infant Child, Preschool Cross Sectional Studies Molecular Biology Descriptive Statistics Feces Microbiology Polymerase Chain Reaction Campylobacter Coinfection Public Health Disease Susceptibility Sex Factors Escherichia Coli Geographic Factors Water Supply Hygiene Infant, Newborn: birth-1 month Infant: 1-23 months Child, Preschool: 2-5 years Male Female ab: Infectious diarrhea remains a widespread, contagious illness, posing severe challenges in developing countries. The aim of the study is to determine the molecular epidemiology of bacterial enteric pathogens associated with diarrhea in children under five years in Lagos State. A total of 268 participants were recruited for this study. Fresh stool samples were collected in sterile, DNase-free containers, and samples were stored at -80°C and analysed using the multiplex quantitative PCR (qPCR) method utilising a fluorescent probe. The study found that E. coli (EPEC) was the most frequently detected pathogen in children, found in 34.0%, followed by Campylobacter jejuni (18.2%). Co-infections involving both pathogens were recorded in 5.7% of participants. No cases of Salmonella, Shigella, Vibrio cholerae, or Aeromonas spp. were found, suggesting possible regional pathogen shifts or the effectiveness of local public health measures. The highest prevalence of all three bacterial infections was observed in infants aged 0-1 years, indicating increased vulnerability. Gender analysis showed near-equal E. coli (EPEC) infection rates between males and females, but the difference was statistically significant. Urban areas had the highest burden of infections across all bacterial types, followed by semi-urban areas, while rural areas reported the fewest cases. E. coli (EPEC) was most prevalent among children using borehole and tap water, with Campylobacter jejuni significantly associated with tap and borehole water use. To reduce diarrhea disease, interventions should prioritise exclusive breastfeeding, safe weaning education, improved water safety, and hygiene promotion through focused maternal and child health programs. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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