γ-mangostin from Garcinia mangostana (L.) exerts the antifungal activity against Cryptococcus neoformans by inhibiting ribosome biogenesis.
The pericarp of Garcinia mangostana (L.) has been traditionally used in Southeast Asia and South China to alleviate diarrhea, regulate gastrointestinal functions, and promote wound healing. It is rich in xanthones, which exhibit significant antimicrobial activity. However, current research on the an...
| Publicado en: | Journal of Ethnopharmacology Vol. 358 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Mar2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=190465806&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 190465806 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: Mar2026 vid: 358 pid: 1004 pub: Elsevier B.V. artinfo: ui: 190465806 190465806 190465806 10.1016/j.jep.2025.120986 190465806 ppct: 1 formats: tig: atl: γ-mangostin from Garcinia mangostana (L.) exerts the antifungal activity against Cryptococcus neoformans by inhibiting ribosome biogenesis. aug: au: Li, Xiancai Chen, Shaohua Wu, Yaodan Liu, Fen Jumai, Aikebaier Zhu, Baojun Xiong, Binghong Qiu, Sheng-Xiang affil: Horticulture Research Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China sug: subj: Cryptococcosis Drug Therapy Plant Extracts Pharmacodynamics Heterocyclic Compounds Therapeutic Use Heterocyclic Compounds Pharmacodynamics Antifungal Agents Pharmacodynamics Antifungal Agents Analysis RNA Drug Effects Human Descriptive Statistics Antifungal Agents Metabolism Biofilms Drug Effects Phytochemicals Microbial Culture and Sensitivity Tests RNA Metabolism Fluconazole Administration and Dosage Signal Transduction Amphotericin B Administration and Dosage ab: The pericarp of Garcinia mangostana (L.) has been traditionally used in Southeast Asia and South China to alleviate diarrhea, regulate gastrointestinal functions, and promote wound healing. It is rich in xanthones, which exhibit significant antimicrobial activity. However, current research on the antimicrobial properties of mangosteen pericarp has primarily concentrated on the antibacterial effects of the xanthone compound α-mangostin, while studies on the antifungal activities of xanthones remain relatively limited. The current study aimed to extract pure compounds from mangosteen pericarp and conducted antifungal activity screening against Cryptococcus neoformans , along with an analysis of the antifungal potential and related mechanisms of action of the most potent antifungal compound. The chemical constituents of G. mangostana pericarp were isolated and identified by phytochemical study and spectroscopic data. The antifungal potential of γ-mangostin was analyzed through minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) tests, structure-activity relationship (SAR) analysis, growth curves, time-kill curves, electron microscopy, biofilm eradication and capsule formation inhibition capacity, as well as a Caenorhabditis elegans infection model treatment. Its antifungal mechanism was investigated via transcriptomic and metabolomic analyses, along with corresponding validation experiments. A total of 33 monomeric compounds were isolated from the mangosteen pericarp extract, primarily xanthone derivatives. Among them, γ-mangostin exhibited the strongest antifungal activity, with MIC and MFC values of 4 μM and 16 μM, respectively. Growth curve, time-kill kinetics, and electron microscopy experiments demonstrated that γ-mangostin exerted significant and sustained antifungal effects within 24 h. At a concentration of 4 μM, γ-mangostin significantly eradicated biofilms and inhibited capsule formation. In a C. elegans infection model, 4 μM γ-mangostin showed notable therapeutic efficacy. Furthermore, γ-mangostin displayed hemolytic activity comparable to the commercial antibiotics fluconazole and amphotericin B at concentrations up to 64 μM, with no adverse effects on nematode lifespan. Transcriptomic and metabolomic analyses revealed that γ-mangostin suppressed the ribosome biogenesis signaling pathway. qPCR analysis indicated that γ-mangostin downregulated key genes (NMD3 , NOB1 , NAT10 , and NHP2) in the ribosome biogenesis pathway. Protein content assays further confirmed that γ-mangostin significantly reduced protein accumulation in C. neoformans. γ-mangostin holds potential application value in the treatment of infections caused by C. neoformans. [Display omitted] pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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