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Diosgenin Ameliorates Cardiac Function following Myocardial Ischemia Through Angiogenic and Anti-Fibrotic Properties; An Experimental Study.

Introduction: Angiogenesis through restoration of blood supply to the ischemic myocardium is a pivotal process that contributes to cardiac repair and leads to improvement of myocardial function. This study was conducted to evaluate cardioprotective effects of Diosgenin against myocardial infarction...

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Published in:Archives of Academic Emergency Medicine Vol. 13; no. 1; pp. 1 - 10
Main Authors: Rakhshan, Kamran, Mohammadkhanizadeh, Ali, Saberi Pirouz, Mahdi, Azizi, Yaser
Format: research Journal Article
Published: Shahid Beheshti University of Medical Sciences 2025
Online Access:View this record in EBSCOhost
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      jtl: Archives of Academic Emergency Medicine
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      dt: 2025
      vid: 13
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      pub: Shahid Beheshti University of Medical Sciences
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        10.22037/aaemj.v13i1.2483
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        atl: Diosgenin Ameliorates Cardiac Function following Myocardial Ischemia Through Angiogenic and Anti-Fibrotic Properties; An Experimental Study.
      aug:
        au:
          Rakhshan, Kamran
          Mohammadkhanizadeh, Ali
          Saberi Pirouz, Mahdi
          Azizi, Yaser
        affil: Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
      sug:
        subj:
          Myocardial Ischemia Drug Therapy
          Fibrosis Drug Therapy
          Plant Extracts Therapeutic Use
          Plant Extracts Pharmacodynamics
          Steroids Therapeutic Use
          Steroids Pharmacodynamics
          Neovascularization, Physiologic Drug Effects
          Cardiac Output Drug Effects
          Oxidative Stress Drug Effects
          Animal Studies
          Rats
          Experimental Studies
          Echocardiography
          Heart Failure Prevention and Control
          Biological Markers
          Histological Techniques
          Enzyme-Linked Immunosorbent Assay
          Creatine Kinase
          Lactate Dehydrogenase
          Superoxide Dismutase
          Malondialdehyde
          Glutathione
          Analysis of Variance
          Post Hoc Analysis
          Statistical Significance
          Data Analysis Software
          Descriptive Statistics
          Funding Source
      ab: Introduction: Angiogenesis through restoration of blood supply to the ischemic myocardium is a pivotal process that contributes to cardiac repair and leads to improvement of myocardial function. This study was conducted to evaluate cardioprotective effects of Diosgenin against myocardial infarction (MI) with focus on angiogenesis, myocardial fibrosis, and oxidative stress. Methods: 4 groups of male Wistar rats were considered for this study: (1) sham, (2) MI, (3) MI+Vehicle and (4) MI+Diosgenin. MI model was created by occluding left anterior descending (LAD) artery for 30 minutes and reperfusion was established for 14 days by opening this artery. Diosgenin (50 mg/kg) was given orally to the rats for 21 days (from 7 days before MI induction until the end of the 14-day reperfusion period). Cardiac injury markers including troponin I, creatine kinase-MB (CK-MB), and lactate dehydrogenase (LDH) were measured using enzyme-linked immunosorbent assay (ELISA), same as cardiac stress oxidative markers (superoxide dismutase (SOD), Malondialdehyde (MDA), reduced glutathione (GSH)). Echocardiography was used to measure heart function parameters and myocardial fibrosis was assessed via a specific tissue staining named Massons trichrome. Blood vessel staining kit was used to assess left ventricular angiogenesis. Results: Ischemia-reperfusion injury increased serum levels of troponin I, CK-MB and LDH, as well as cardiac malondialdehyde (MDA) and myocardial fibrosis. MI also decreased myocardial function (Ejection fraction (EF)% and Fractional shortening (FS)%) and Diosgenin treatment reversed these parameters. Capillary density as marker of angiogenesis significantly increased in all of MI groups. However, development of angiogenesis was significantly higher in Diosgenin group compared with MI group. Conclusion: Diosgenin exerts cardioprotective effects against ischemia-reperfusion injury by strengthening cardiac antioxidant defense and reducing deposition of collagen fibers. It seems that the strengthening of angiogenesis in heart tissue is one of the main mechanisms of Diosgenin to increase the heart’s resistance against ischemia.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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