Development of tobacco chloroplast vector with novel insertion sites for dengue virus (DENV) antigens.
Dengue virus (DENV) is a substantial global health threat with no specific antiviral treatment available. The development of effective vaccines is crucial to combat DENV infections. Plant-based expression systems, such as tobacco chloroplasts, have emerged as promising platforms for the production o...
| Publicado en: | Protoplasma Vol. 263; no. 1; pp. 161 - 168 |
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| Autores principales: | , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jan2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=190820860&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 190820860 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Jan2026 vid: 263 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 190820860 186695470 10.1007/s00709-025-02087-5 190820860 ppf: 161 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Development of tobacco chloroplast vector with novel insertion sites for dengue virus (DENV) antigens. aug: au: Almohaimeed, Hailah M. Assiri, Rasha Aggad, Waheeb S. ALsharafa, Meshal M. Faloudah, Sara F. Abdulfattah, Ahmed M. Alsulaimani, Fayez Basri, Ahmed M. affil: https://ror.org/05b0cyh02 Department of Basic Science, College of Medicine, Princess Nourah bint Abdulrahman University, P. O. Box 84428, 11671, Riyadh, Saudi Arabia sug: ab: Dengue virus (DENV) is a substantial global health threat with no specific antiviral treatment available. The development of effective vaccines is crucial to combat DENV infections. Plant-based expression systems, such as tobacco chloroplasts, have emerged as promising platforms for the production of DENV antigens. In this research article, we describe the construction of a tobacco chloroplast vector with novel insertion sites for DENV-1 protein III. Our present study successfully demonstrated the production of the envelope protein structural domain III (EDIII) dengue virus antigen in tobacco chloroplasts. We obtained transformed tobacco lines that show the expression of EDIII dengue antigen and were confirmed through Southern blotting analysis. Furthermore, the expression of EDIII antigen was further confirmed via immunoblotting and quantified. These findings suggest that tobacco chloroplast engineering holds promise as a method for future manufacture of an oral dengue boost vaccine that is safe, affordable, and effective, contributing to the global management and control of this particular dengue infection. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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