Next-generation Sequencing for Outbreak Investigation in the Clinical Microbiology Laboratory.
With the advent of next-generation sequencing technologies, genomic investigations of pathogen outbreaks are now possible at unprecedented resolution. Prior methods requiring highly pathogen-specific protocols can now be replaced with a single universal protocol for whole-genome sequencing. Clinical...
| Publicado en: | Clinical Laboratory Science Vol. 32; no. 2; pp. 70 - 78 |
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| Autores principales: | , |
| Formato: | pictorial review tables/charts Journal Article |
| Publicado: |
American Society for Clinical Laboratory Science
Spring2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=190973739&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 190973739 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0894959X IKL jtl: Clinical Laboratory Science issn: 0894959X maglogo: N pubinfo: dt: Spring2019 vid: 32 iid: 2 pid: 13703 pub: American Society for Clinical Laboratory Science place: Dundee, Michigan artinfo: ui: 190973739 190973739 190973739 190973739 ppf: 70 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Next-generation Sequencing for Outbreak Investigation in the Clinical Microbiology Laboratory. aug: au: DOUGHERTY, CAITLIN E. GRAF, ERIN affil: Children's Hospital of Philadelphia sug: subj: Sequence Analysis Methods Disease Outbreaks Diagnosis, Laboratory Communicable Diseases Drug Resistance, Microbial Microbiological Techniques Behavioral Objectives Genome Intensive Care Units, Neonatal Methicillin-Resistant Staphylococcus Aureus Enterococcus Reverse Transcriptase Polymerase Chain Reaction Workforce Electrophoresis, Gel, Pulsed-Field Cost Savings ab: With the advent of next-generation sequencing technologies, genomic investigations of pathogen outbreaks are now possible at unprecedented resolution. Prior methods requiring highly pathogen-specific protocols can now be replaced with a single universal protocol for whole-genome sequencing. Clinical applications have shown improvement in our understanding of pathogen transmission dynamics from the hospital to global level. However, there are several barriers to routine implementation in clinical microbiology laboratories. These include financial support for a nonbillable test; workforce training needs, particularly for highly complex data analysis methods; and lack of Food and Drug Administration-approved methods requiring extensive laboratory validation. pubtype: Academic Journal doctype: pictorial review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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