Use of Diagnostic Metagenomics in the Clinical Microbiology Laboratory.
Next-generation sequencing (NGS)-based assays have recently entered the realm of the clinical microbiology laboratory's capacity, providing exciting potential for improvement in infectious disease detection and identification. There are many diagnostic applications of NGS, such as targeted or amplic...
| Publicado en: | Clinical Laboratory Science Vol. 32; no. 2; pp. 78 - 85 |
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| Autor principal: | |
| Formato: | pictorial review tables/charts Journal Article |
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American Society for Clinical Laboratory Science
Spring2019
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=190973740&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 190973740 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0894959X IKL jtl: Clinical Laboratory Science issn: 0894959X maglogo: N pubinfo: dt: Spring2019 vid: 32 iid: 2 pid: 13703 pub: American Society for Clinical Laboratory Science place: Dundee, Michigan artinfo: ui: 190973740 190973740 190973740 190973740 ppf: 78 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Use of Diagnostic Metagenomics in the Clinical Microbiology Laboratory. aug: au: MITCHELL, STEPHANIE L. affil: University of Pittsburg School of Medicine sug: subj: Genome Diagnosis, Laboratory Methods Microbiology Education Clinical Laboratories Sequence Analysis Communicable Diseases Diagnosis Technology, Medical DNA RNA Bioinformatics Specimen Handling Quality Control (Technology) Clinical Laboratory Information Systems Personnel Staffing and Scheduling Information Systems Clinical Competence Laboratory Personnel Education ab: Next-generation sequencing (NGS)-based assays have recently entered the realm of the clinical microbiology laboratory's capacity, providing exciting potential for improvement in infectious disease detection and identification. There are many diagnostic applications of NGS, such as targeted or amplicon NGS and metagenomic NGS (mNGS). mNGS has received the most attention for diagnostics because of its unbiased nature and "hypothesis-free" testing approach. Although mNGS may have improved pathogen detection compared with conventional culture-based testing and has shown clinical utility in some specific cases, the application of this technology is still investigational, and many barriers and limitations remain to be overcome. This review will cover both the advantages and limitations of mNGS and address the need for and incorporation of new technologist skillsets in the clinical microbiology laboratory to successfully implement mNGS diagnostics. pubtype: Academic Journal doctype: pictorial review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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