The alterations in brain network functional gradients and dynamic functional connectivity in Alzheimer's disease: a resting-state fMRI study.

Background and purpose: Alzheimer's disease (AD), the most common form of dementia worldwide, is characterized by progressive cognitive decline. Extensive evidence from dynamic functional connectivity (dFC) studies has demonstrated unstable functional states, reduced network flexibility, and impaire...

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Publicado en:Frontiers in Aging Neuroscience pp. 1 - 17
Autores principales: Liu, Benqin, Chen, Chunbing, Cai, Pan, Zhang, Jiaren, Yang, Li, Chen, Xu, Ma, Xuejin, Jiang, Xuetao, Zhang, Anjie, Song, Linfeng, Jiang, Lin
Formato: pictorial research tables/charts Journal Article
Publicado: Frontiers Media S.A. 2026
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2026
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      pub: Frontiers Media S.A.
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        10.3389/fnagi.2025.1716076
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        atl: The alterations in brain network functional gradients and dynamic functional connectivity in Alzheimer's disease: a resting-state fMRI study.
      aug:
        au:
          Liu, Benqin
          Chen, Chunbing
          Cai, Pan
          Zhang, Jiaren
          Yang, Li
          Chen, Xu
          Ma, Xuejin
          Jiang, Xuetao
          Zhang, Anjie
          Song, Linfeng
          Jiang, Lin
        affil: Department of Radiology, The Third Affiliated Hospital of Zunyi Medical University (The First People's Hospital of Zunyi), Zunyi, China
      sug:
        subj:
          Alzheimer's Disease
          Brain Physiology
          Large-Scale Brain Networks
          Functional Connectivity
          Brain Mapping
          Cognition Disorders
          Machine Learning
          Human
          Magnetic Resonance Imaging
          Diagnosis, Brain Methods
          T-Tests
          Biological Markers
          Sensitivity and Specificity
          Funding Source
          Male
          Female
          Aged
          Middle Age
          Scales
          Pearson's Correlation Coefficient
          Fisher's Exact Test
          ROC Curve
          Aged: 65+ years
          Middle Aged: 45-64 years
          Male
          Female
      ab: Background and purpose: Alzheimer's disease (AD), the most common form of dementia worldwide, is characterized by progressive cognitive decline. Extensive evidence from dynamic functional connectivity (dFC) studies has demonstrated unstable functional states, reduced network flexibility, and impaired transitions between large-scale neurocognitive networks across the AD continuum. However, how these temporal abnormalities are embedded within the hierarchical spatial organization of brain networks, as captured by functional gradients (FG), and whether combined FG-dFC metrics can provide mechanistically interpretable and potentially sensitive imaging biomarkers, remain to be elucidated. Methods: This study enrolled 46 AD patients who were diagnosed according to the Amyloid/Tau/Neurodegeneration (ATN) biological diagnostic framework and 37 age- and sex-matched healthy controls (HC). All participants underwent resting-state fMRI. Functional gradients were derived using connectivity similarity matrices and diffusion embedding (aligned and standardized), while dFC was estimated with a sliding window approach and clustered into four recurrent states. Group differences were assessed with two-sample t -tests with Gaussian Random Field (GRF) correction. Correlation analyses included ATN biomarkers and cognitive scores. A linear support vector machine (SVM) with leave-one-out cross-validation evaluated classification performance based on significant FG features. Results: Compared to the healthy controls, AD patients exhibited widespread FG alterations between regions of the Default Mode Network (DMN) and the Sensorimotor Network (SMN). In the first gradient DMN, the left precuneus showed reduced gradient scores, whereas the right medial superior frontal gyrus and bilateral angular gyri were increased. In the first gradient of the SMN, the right supplementary motor area increased while bilateral superior temporal gyri decreased. Second-gradient reductions were confined to two regions: the left postcentral gyrus (SMN) and left middle occipital gyrus (visual network, VIS). The right medial superior frontal gyrus first-gradient score correlated negatively with T-Tau (r = −0.50, P = 0.006) and age (r = −0.36, P = 0.02); the right angular gyrus correlated negatively with age (r = −0.29, P = 0.04); the left precuneus correlated positively with age (r = 0.38, P = 0.009). dFC revealed four recurrent states (27.59, 17.67, 28.27, 26.47% of total occurrences). Relative to HC, AD showed higher FT and MDT in states 1–2 and lower scores in state 3, with NT unchanged, alongside state-dependent bidirectional connectivity changes (fronto-insular-sensorimotor increases; DMN-temporal and visuo-auditory decreases). The SVM achieved an AUC of 0.776, sensitivity 78.26%, specificity 67.57%, and accuracy 73.49%, with the right superior temporal gyrus within SMN first-gradient contributing most. Conclusion: AD is characterized by macro-scale hierarchical disorganization centered on the principal functional gradient, accompanied by reduced cross-state flexibility and state-dependent connectivity abnormalities. The combined functional gradient-dynamic functional connectivity (FG-dFC) analysis provides complementary spatiotemporal insights and reveals imaging features associated with T-Tau levels and age, offering new perspectives on the neuropathological mechanisms of AD and potential imaging biomarkers. Moreover, these network topology and dynamic connectivity metrics may prove useful for monitoring disease progression, evaluating treatment effects, and stratifying patients in future clinical and interventional studies.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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