Automated Mycobacterium tuberculosis Detection in Multivariant Digitized Ziehl–Neelsen Staining Using Faster R‐CNN Method.

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and remains a major public health concern in Indonesia. One of the most widely used diagnostic methods is the microscopic examination of sputum smears stained using the Ziehl–Neelsen technique. However, manual identifica...

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Publicado en:International Journal of Biomedical Imaging Vol. 2026; pp. 1 - 11
Autores principales: Rulaningtyas, Riries, Bilhaq, Fashalli Giovi, Kusumaningrum, Deby, Eric, Ronald, Ittaqillah, Sayyidul Istighfar, Trilaksana, Herri, Widhyatmoko, Dicky Bagus, Joseph, Annie Anak, Ahmad, Irfan
Formato: equations & formulas pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 1/21/2026
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 1/21/2026
      vid: 2026
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/ijbi/6692222
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        atl: Automated Mycobacterium tuberculosis Detection in Multivariant Digitized Ziehl–Neelsen Staining Using Faster R‐CNN Method.
      aug:
        au:
          Rulaningtyas, Riries
          Bilhaq, Fashalli Giovi
          Kusumaningrum, Deby
          Eric, Ronald
          Ittaqillah, Sayyidul Istighfar
          Trilaksana, Herri
          Widhyatmoko, Dicky Bagus
          Joseph, Annie Anak
          Ahmad, Irfan
        affil: Biomedical Engineering Study Program,, Department of Physics,, Faculty of Science and Technology,, Universitas Airlangga,, Surabaya, East Java,, Indonesia, unair.ac.id
      sug:
        subj:
          Automation, Laboratory
          Mycobacterium Tuberculosis Analysis
          Tuberculosis Diagnosis
          Staining and Labeling
          Convolutional Neural Networks
          Deep Learning
          Detection Algorithms
          Diagnosis, Computer Assisted
          Sputum Microbiology
          Human
          Indonesia
          Software
          Data Analysis, Computer Assisted
          Data Collection, Computer Assisted
          Sensitivity and Specificity Evaluation
          Precision Evaluation
          Comparative Studies
          Programming Languages
          Experimental Studies
          Microscopy
          Descriptive Statistics
      ab: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and remains a major public health concern in Indonesia. One of the most widely used diagnostic methods is the microscopic examination of sputum smears stained using the Ziehl–Neelsen technique. However, manual identification of TB bacteria presents significant challenges, particularly due to staining thickness variations that lead to inconsistent color intensities, making visual detection difficult and often subjective. This study is aimed at developing an automated TB bacteria detection system using deep learning, specifically the Faster R‐CNN algorithm with ResNet‐50 layers. The system is implemented using the Python programming language and the TensorFlow Object Detection API. We incorporated data augmentation in the form of random rotation, random flipping, and color processing such as hue variation, saturation stretching, brightness stretching, and exposure stretching. Experimental results show that the proposed model achieves an accuracy of 88%, with a precision of 94%, recall of 93%, and an F1‐score of 94%. The model outputs annotated images indicating the locations of TB bacteria, which can assist medical professionals in the diagnostic process. These findings demonstrate the potential of deep learning–based approaches in automating TB detection, particularly in healthcare settings with limited human resources.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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