Antitrypanosomal Activity and Molecular Docking Studies of Lobetyolin From Lobelia rhynchopetalum Hemsl. Root Extract Against Trypanosoma congolense Field Isolates.
Currently available drugs for trypanosomiasis are few, and their use is limited by toxicity and growing resistance. This highlights the need for safer and more effective alternatives. In this study, the in vitro and in vivo antitrypanosomal activities of an 80% methanol root extract of Lobelia rhync...
| Publicado en: | BioMed Research International Vol. 2026; pp. 1 - 16 |
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| Autores principales: | , , , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/4/2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=191376539&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 191376539 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/4/2026 vid: 2026 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 191376539 191376539 191376539 10.1155/bmri/2214310 191376539 ppf: 1 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Antitrypanosomal Activity and Molecular Docking Studies of Lobetyolin From Lobelia rhynchopetalum Hemsl. Root Extract Against Trypanosoma congolense Field Isolates. aug: au: Yimer, Selamawit Tewelde, Eyael Bisrat, Daniel Tadesse, Solomon Yeshak, Mariamawit Y. Shivahare, Rahul affil: Department of Pharmacognosy,, School of Pharmacy,, College of Health Sciences,, Bahir Dar University,, Bahir Dar, Ethiopia, bdu.edu.et sug: subj: Protozoa Drug Effects Plant Extracts Pharmacodynamics Plant Roots Antiprotozoal Agents Pharmacodynamics Phytochemicals Pharmacodynamics Trypanosomiasis Drug Therapy Mice Animal Studies Models, Biological Molecular Docking Simulation In Vitro Studies In Vivo Studies Parasitemia Prevention and Control Oxidation-Reduction Drug Effects Plant Extracts Administration and Dosage Phytochemicals Administration and Dosage Ethiopia Descriptive Statistics Data Analysis Software One-Way Analysis of Variance Post Hoc Analysis Funding Source ab: Currently available drugs for trypanosomiasis are few, and their use is limited by toxicity and growing resistance. This highlights the need for safer and more effective alternatives. In this study, the in vitro and in vivo antitrypanosomal activities of an 80% methanol root extract of Lobelia rhynchopetalum and its major constituent were evaluated against Trypanosoma congolense field isolates. Phytochemical separation of the extract yielded lobetyolin (a polyacetylene compound), confirmed through spectroscopic analysis. At 4 mg/mL, both the crude extract and lobetyolin inhibited parasite motility, with lobetyolin acting slightly faster (25 min) than the extract (30 min). The blood incubation assay demonstrated dose‐dependent protection, with 4 mg/mL of DA, the lobetyolin‐rich extract, or pure lobetyolin preventing infection, while lower doses only delayed parasitemia. In vivo testing showed that lobetyolin at 100 mg/kg exhibited stronger activity than the crude extract at 400 mg/kg. Molecular docking demonstrated that lobetyolin binds strongly to the homology‐modeled trypanothione reductase (TR) enzyme of T. congolense, achieving a Glide score of −8.002 kcal/mol, which is close to that of the native ligand (−8.307 kcal/mol). This suggests that lobetyolin may inhibit TR, thereby disrupting the parasite′s redox balance essential for survival. Overall, these findings provide the first evidence supporting the antitrypanosomal activity of L. rhynchopetalum root extract and lobetyolin, providing scientific support to the plant′s traditional use. Further studies are needed to fully validate its therapeutic potential. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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