An Investigation of Colistin Heteroresistance in Klebsiella pneumoniae Isolates in Iran.
Background: According to the World Health Organization 2024 bacterial priority pathogens list, carbapenem‐resistant Enterobacterales (CRE) were listed among the critical priority pathogens. Heteroresistance (i.e., a bacterial isolate that appears susceptible but harbors resistant subpopulations) rep...
| Publicado en: | BioMed Research International Vol. 2026; pp. 1 - 9 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/4/2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=191376541&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 191376541 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/4/2026 vid: 2026 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 191376541 191376541 191376541 10.1155/bmri/4239177 191376541 ppf: 1 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: An Investigation of Colistin Heteroresistance in Klebsiella pneumoniae Isolates in Iran. aug: au: Rad, Zohreh Riahi Rad, Zahra Riahi Goudarzi, Hossein Goudarzi, Mehdi Javidi, Mohsen Sharahi, Javad Yasbolaghi Kargar, Masoud Hashemi, Ali Banerjee, Baisakhi affil: Department of Microbiology,, School of Medicine,, Shahid Beheshti University of Medical Sciences,, Tehran, Iran, sbmu.ac.ir sug: subj: Drug Resistance, Microbial Iran Klebsiella Colistin Therapeutic Use Human Iran Microbial Culture and Sensitivity Tests Male Female Descriptive Statistics Carbapenems Maps Funding Source Male Female ab: Background: According to the World Health Organization 2024 bacterial priority pathogens list, carbapenem‐resistant Enterobacterales (CRE) were listed among the critical priority pathogens. Heteroresistance (i.e., a bacterial isolate that appears susceptible but harbors resistant subpopulations) represents a challenge in traditional laboratory testing, which may lead to treatment failure with colistin. This phenomenon has been studied in many bacteria, including K. pneumoniae. To our knowledge, this is the first report of colistin heteroresistance in K. pneumoniae isolates using population analysis profiles (PAPs) in Iran. Methods: Between 2019 and 2020, 100 K. pneumoniae isolates were collected from various samples of hospitalized patients in Iran. This study primarily determined antibiotic resistance by antimicrobial susceptibility testing. Thereafter, the prevalence of colistin heteroresistance in K. pneumoniae isolates was evaluated by the PAP test. Heteroresistant isolates were typed by multilocus sequence typing (MLST). Results: The MIC test showed that 79 (79%) of the 100 K. pneumoniae isolates were susceptible to colistin. Overall, 2/79 colistin‐susceptible isolates were classified as heteroresistant isolates by the PAP method, with a colistin MIC of 0.5 μg/ml. Importantly, after 5 serial passaging on colistin‐free plates, there was no increase in the MIC of the colistin‐resistant subpopulations, showing that heteroresistance cases were unstable. MLST revealed that heteroresistant isolates belong to ST377 and ST15. Conclusions: In conclusion, the current study contributes to our understanding of the challenges posed by heteroresistant isolates in clinical laboratories. Since heteroresistant isolates may be misidentified as susceptible by standard tests, these findings raise concerns regarding the interpretation of colistin susceptibility results. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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