Correlation between a real-time bioparticle detection device and a traditional microbiological active air sampler monitoring air quality in an operating room during elective arthroplasty surgery: a prospective feasibility study.
Background and purpose — The standard method for controlling operating room (OR) air quality is measuring bacteria-carrying particles per volume unit of air: colony forming units (CFU/m³). The result takes at least 2 days after sampling. Another method is real-time measurements of fluorescing biopar...
| Publicado en: | Acta Orthopaedica Vol. 96; pp. 176 - 182 |
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| Autores principales: | , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Medical Journals Sweden AB
2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=191461352&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 191461352 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17453674 1BVL jtl: Acta Orthopaedica issn: 17453674 maglogo: N pubinfo: dt: 2025 vid: 96 pid: 59195 pub: Medical Journals Sweden AB artinfo: ui: 191461352 191461352 191461352 10.2340/17453674.2025.43002 191461352 ppf: 176 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Correlation between a real-time bioparticle detection device and a traditional microbiological active air sampler monitoring air quality in an operating room during elective arthroplasty surgery: a prospective feasibility study. aug: au: LARSSON, Lise-Lott NORDENADLER, Johan BJÖRLING, Gunilla FELLÄNDER-TSAI, Li LAZARINIS, Stergios LJUNGQVIST, Bengt MATTSSON, Janet REINMÜLLER, Berit BRISMAR, Harald affil: Division of Orthopaedics and Biotechnology, CLINTEC, Karolinska Institutet, Stockholm, Sweden. sug: subj: Air Microbiology Equipment and Supplies Air Pollution, Indoor Analysis Operating Rooms Arthroplasty Surgery, Elective Environmental Monitoring Intraoperative Monitoring Human Funding Source Male Female Prospective Studies Pilot Studies Academic Medical Centers Spearman's Rank Correlation Coefficient Confidence Intervals Linear Regression Descriptive Statistics Data Analysis Software Diathermy Utilization Post Hoc Analysis Comparative Studies Ventilation Male Female ab: Background and purpose — The standard method for controlling operating room (OR) air quality is measuring bacteria-carrying particles per volume unit of air: colony forming units (CFU/m³). The result takes at least 2 days after sampling. Another method is real-time measurements of fluorescing bioparticles per unit volume of air (FBP/dm³). We aimed to compare simultaneous measurements of FBP/50 dm³ and CFU/m³ during ongoing arthroplasty surgery. Methods — 18 arthroplasties were performed in a modern OR with turbulent mixed airflow ventilation. The sampling heads of a BioAerosol Monitoring System (BAMS) and a microbiological active air sampler (Sartorius MD8 Air Sampler) were placed next to each other, and 6 parallel 10-minute registrations of FBP/50 dm³ and CFU/m³ were performed for each surgery. Parallel measurements were plotted against each other, Passing–Bablok nonparametric linear regression was performed, and the Spearman correlation coefficient (r) was calculated. Results — The r between FBP ≥ 3 μm/50 dm³ and CFU/ m³ sampled for 96 x 10-minute intervals, was 0.70 (95% confidence interval [CI] 0.57–0.79). In the 25th percentile with the lowest 10-minute FBP ≥ 3μm/50 dm³, there were no CFU measurements with ≥ 10 and 4% with ≥ 5 CFU/m³. In the 75th percentile with the highest 10-minute FBP ≥ 3 μm/50 dm³, there were 58% CFU measurements with ≥ 10 and 88% with ≥ 5 CFU/m³. The r between FBP ≥ 3 μm/50 dm³ and CFU/m³ means sampled during 18 operations was 0.87 (CI 0.68–0.95). Conclusion — Low FBP ≥ 3 μm/50 dm³ measured by BAMS indicates low CFU/m³; conversely, high FBP ≥ 3 μm/50 dm³ indicates high CFU/m³. Real-time measurements of FBP ≥ 3 μm/50 dm³ can be used as a supplement to CFU/m³ monitoring OR air bacterial load. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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