The DREAM and MEC NuRD complexes reinforce SPR-5/MET-2 maternal reprogramming to maintain the germline–soma distinction.
The proper coordination of transcription factors, ATP dependent chromatin remodelers, and histone modifications is essential for tissue-specific gene expression, but how gene expression is regulated at these different levels is not well understood. In Caenorhabditis elegans , H3K4 methylation that i...
| Publicado en: | Genetics Vol. 232; no. 3; pp. 1 - 13 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Mar2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=192264875&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 192264875 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00166731 GNT jtl: Genetics issn: 00166731 maglogo: N pubinfo: dt: Mar2026 vid: 232 iid: 3 pid: 622 pub: Oxford University Press / USA artinfo: ui: 192264875 192264875 192264875 10.1093/genetics/iyag008 192264875 ppf: 1 ppct: 12 formats: tig: atl: The DREAM and MEC NuRD complexes reinforce SPR-5/MET-2 maternal reprogramming to maintain the germline–soma distinction. aug: au: Chavez, Sindy R Dozier, Jazmin Gosrani, Saahj P Nguyen, Sandra K Brockett, Jovan S Blancher, Sarah D Morgan-Benitez, Sydney L Rodriguez, Juan D Birol, Onur Reeves, Monica N affil: Department of Cell Biology, Emory University School of Medicine, Emory University, Atlanta, GA 30322, United States sug: subj: Surface Plasmon Resonance Enzymes Metabolism Germ Cells Proteins Metabolism Cell Differentiation Caenorhabditis Elegans Animal Studies Larva RNA Descriptive Statistics Gene Expression Fetal Development Oxidoreductases Metabolism Transferases Metabolism Histones Metabolism Funding Source ab: The proper coordination of transcription factors, ATP dependent chromatin remodelers, and histone modifications is essential for tissue-specific gene expression, but how gene expression is regulated at these different levels is not well understood. In Caenorhabditis elegans , H3K4 methylation that is acquired in the germline is reprogrammed at fertilization by the H3K4me1/2 demethylase SPR-5 /LSD1/KDM1A and the H3K9 methyltransferase MET-2 /SETDB1/KMT2E. SPR-5 / MET-2 maternal reprogramming is required to help establish the germline–soma distinction and prevent developmental delay by preventing inherited H3K4 methylation from inappropriately maintaining germline gene expression in somatic tissues. To determine if the DREAM transcriptional repressor complex and the MEC NuRD ATP dependent nucleosome remodeling and histone deacetylase complex function to reinforce SPR-5 / MET-2 maternal reprogramming, we asked if loss of these complexes affects the ectopic germline transcription and developmental delay in spr-5 ; met-2 double mutants. We find that knocking down the DREAM or MEC NuRD complexes specifically exacerbates the developmental delay and the ectopic expression of germline genes in the soma caused by loss of SPR-5 and MET-2. In addition, the DREAM and MEC NuRD complexes bind together at SPR-5 / MET-2 reprogramming targets. These data suggest that the transcriptional repression of DREAM and the ATP dependent chromatin remodeling and deacetylation activities of the MEC NuRD complex are required somatically to reinforce maternal histone reprogramming by SPR-5 / MET-2. Thus, these data provide a novel example of how gene regulation is coordinated at multiple levels to maintain the germline–soma distinction and ensure proper development. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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