From Smear to Sequence: Using Thyroid Cytology Smear Slides for ThyroSeq Molecular Analysis and Clinicopathologic Insights From 61 Tested Nodules.
Context.--In the absence of conventional testing media, thyroid cytology smear slides may be used for molecular analysis of nodules with indeterminate cytology results. Objective.--To present solutions for overcoming challenges of ThyroSeq testing using smear slides and report findings from tested n...
| Publicado en: | Archives of Pathology & Laboratory Medicine Vol. 150; no. 3; pp. 202 - 210 |
|---|---|
| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
College of American Pathologists
Mar2026
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=192327254&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 192327254 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00039985 1FS jtl: Archives of Pathology & Laboratory Medicine issn: 00039985 maglogo: N pubinfo: dt: Mar2026 vid: 150 iid: 3 pid: 2550 pub: College of American Pathologists place: Northfield, Illinois artinfo: ui: 192327254 192327254 192327254 10.5858/arpa.2025-0115-OA 192327254 ppf: 202 ppct: 8 formats: fmt: @attributes: type: P tig: atl: From Smear to Sequence: Using Thyroid Cytology Smear Slides for ThyroSeq Molecular Analysis and Clinicopathologic Insights From 61 Tested Nodules. aug: au: Trabzonlu, Levent Elliott, Caroline Fisch, Adam S. Chebib, Ivan A. Madrigal, Emilio affil: Department of Pathology, Massachusetts General Hospital, Boston sug: subj: Thyroid Nodule Pathology Thyroid Neoplasms Diagnosis Cancer Screening Methods Biopsy, Needle Molecular Diagnostic Techniques Sequence Analysis Human Male Female Adult Middle Age Aged Aged, 80 and Over Massachusetts Hospitals, Public Retrospective Design Record Review Medical Records Prospective Studies Mutation Gene Expression Comparative Studies Descriptive Statistics Data Analysis Software DNA RNA Thyroid Neoplasms Familial and Genetic Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Aged, 80 & over Male Female ab: Context.--In the absence of conventional testing media, thyroid cytology smear slides may be used for molecular analysis of nodules with indeterminate cytology results. Objective.--To present solutions for overcoming challenges of ThyroSeq testing using smear slides and report findings from tested nodules. Design.--We developed software to parse unstructured ThyroSeq reports for actionable data extraction. To ensure compliance with sample retention requirements, substitute specimen records were created by digitizing smear slides before they were exhausted for molecular analysis.We stream-lined the test send-out process and recorded the clinical, molecular, and pathologic findings of the cases in our cohort. Results.--We submitted 61 thyroid fine-needle aspiration specimens from 59 cases for ThyroSeq testing. All 61 specimens were adequate for DNA analysis, and only 1 was insufficient for RNA analysis. In 8 cases, the smear slide was the only viable sample for molecular testing. A total of 21 specimens (34.4%) had a positive ThyroSeq result. Gene mutations were the most common findings, with 16 mutations detected in 13 "positive" specimens. Additionally, copy number alterations, gene expression alterations, and gene fusions were identified. Conclusions.--This study presents our approach to extending the utility of thyroid cytology smear slides by enabling molecular analysis, particularly when routine sample types are unavailable. High adequacy rates and successful detection of molecular alterations highlight the potential of smear slides in molecular testing, reducing the need for repeated procedures and streamlining care. Effective communication between clinical and cytology teams remains essential to manage the additional workload. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|