Genetic Diversity and Ecological Distribution of Bulinus Populations in Southern Benin Using PCR‐RFLP Analysis.
Schistosomiasis remains a major public health concern in Benin. Freshwater snails of the genus Bulinus serve as intermediate hosts for schistosomes, trematode parasites responsible for bilharzia. The urinary form, caused by Schistosoma haematobium, is the most prevalent and is primarily transmitted...
| Published in: | BioMed Research International Vol. 2026; pp. 1 - 19 |
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| Main Authors: | , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
3/25/2026
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=192505882&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 192505882 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 3/25/2026 vid: 2026 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 192505882 192505882 192505882 10.1155/bmri/5536391 192505882 ppf: 1 ppct: 18 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Genetic Diversity and Ecological Distribution of Bulinus Populations in Southern Benin Using PCR‐RFLP Analysis. aug: au: Alabi, Elokou Do, Aboudou Chabi Nougbode, Jonathan Lehmane, Halfane Sina, Haziz Sedah, Paulin Adjanohoun, Adolphe Sezonlin, Michel Baba-Moussa, Lamine Wesley, Hannah affil: Research Unit in Phylogeography,, Genetics and Evolution,, Faculty of Science and Technology,, University of Abomey-Calavi,, Abomey-Calavi, Benin, uac.bj sug: subj: Schistosomiasis Transmission Mollusca Genetic Variation Polymorphism, Genetic Ecosystem Disease Vectors Prevention and Control Schistosomiasis Prevention and Control Animal Studies Molecular Diagnostic Techniques Epidemiology, Molecular Polymerase Chain Reaction Cross Sectional Studies Sequence Analysis Spectrophotometers Descriptive Statistics Data Analysis Software Schistosomiasis Epidemiology Kruskal-Wallis Test ab: Schistosomiasis remains a major public health concern in Benin. Freshwater snails of the genus Bulinus serve as intermediate hosts for schistosomes, trematode parasites responsible for bilharzia. The urinary form, caused by Schistosoma haematobium, is the most prevalent and is primarily transmitted by Bulinus truncatus and Bulinus globosus, whereas the intestinal form, caused by Schistosoma mansoni, is transmitted by Bulinus forskalii. Effective control strategies require an understanding of the molecular diversity and genetic structure of Bulinus populations. Bulinus samples were collected from six localities in southern Benin: Sô‐Ava, Azowlissè, Aplahoué, Kpinnou, Houéyogbé, and Za‐Kpota. Samples were preserved in absolute alcohol. DNA was extracted, and fragments of the Cox1 and SSU genes were amplified. Molecular diversity was assessed using PCR‐RFLP, with amplified products digested using HaeIII, HinfI, and DdeI restriction enzymes. Molecular variance analysis (AMOVA) was performed to evaluate population structure. AMOVA revealed heterozygosity deficits in Bulinus populations from all six localities and deviations from panmixia in Sô‐Ava, Aplahoué, Houéyogbé, Kpinnou, and Za‐Kpota. Enzymatic digestion indicated monomorphism for B. globosus in Za‐Kpota and Houéyogbé with the SSU gene and for B. truncatus in Sô‐Ava and Azowlissè with the Cox1 gene. Conversely, molecular polymorphism was observed for B. globosus in Sô‐Ava and Aplahoué with the Cox1 gene, whereas B. truncatus in Sô‐Ava and Azowlissè showed identical SSU gene profiles. These findings provide preliminary insights into the molecular diversity and genetic structure of Bulinus populations in southern Benin. The observed genetic variability and structure have implications for understanding the transmission dynamics of S. haematobium and for designing targeted schistosomiasis control strategies. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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