Integrons and Efflux Pump‐Mediated Antibiotic Resistance in Multidrug‐Resistant (MDR) Uropathogenic Escherichia coli.
Objective: Integrons facilitate the acquisition and expression of antibiotic resistance genes, with Classes 1 and 2 commonly found in clinical E. coli. The AcrAB–TolC efflux pump is a major RND system that expels diverse antimicrobial agents and contributes to resistance. This study is aimed at dete...
| Publicado en: | BioMed Research International Vol. 2026; pp. 1 - 9 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
4/2/2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=192727007&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 192727007 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 4/2/2026 vid: 2026 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 192727007 192727007 192727007 10.1155/bmri/2158306 192727007 ppf: 1 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Integrons and Efflux Pump‐Mediated Antibiotic Resistance in Multidrug‐Resistant (MDR) Uropathogenic Escherichia coli. aug: au: Gonbadi, Mohammad Amin Dini, Yasaman Mohammadi, Mohsen Pournajaf, Abazar Halaji, Mehrdad Bright, Richard affil: Student Research Committee,, Babol University of Medical Sciences,, Babol, Iran, mubabol.ac.ir sug: subj: Antibiotics Pharmacodynamics Drug Resistance, Multiple Escherichia Coli Infections Drug Therapy Escherichia Coli Infections Microbiology Bacterial Proteins Drug Effects Urinary Tract Infections Microbiology Urinary Tract Infections Drug Therapy Escherichia Coli Drug Effects Gene Expression Profiling Human Male Female Infant Child, Preschool Child Adolescence Adult Middle Age Aged Aged, 80 and Over Membrane Transport Proteins Drug Effects Microbial Culture and Sensitivity Tests Qualitative Studies Polymerase Chain Reaction Biochemical Phenomena Iran Genotype Descriptive Statistics Data Analysis Software Phenotype Chi Square Test Cross Sectional Studies Biological Assay DNA Physiology Staining and Labeling Genomics Funding Source Spectrophotometry Electrophoresis Infant: 1-23 months Child, Preschool: 2-5 years Child: 6-12 years Adolescent: 13-18 years Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Aged, 80 & over Male Female ab: Objective: Integrons facilitate the acquisition and expression of antibiotic resistance genes, with Classes 1 and 2 commonly found in clinical E. coli. The AcrAB–TolC efflux pump is a major RND system that expels diverse antimicrobial agents and contributes to resistance. This study is aimed at determining the prevalence of Class 1/2 integrons and the AcrAB–TolC efflux pump in UPEC isolates from UTI patients in Babol, Iran. Methods: This cross‐sectional study analyzed 155 UPEC isolates collected from UTI patients in Babol, northern Iran (2021–2023). Isolates were identified using standard biochemical and genotypic methods. Antimicrobial susceptibility was assessed by disk diffusion. Polymerase chain reaction (PCR) was used to detect the presence of integron and efflux pump genes. Statistical analysis was performed using SPSS (Version 16), with significance defined at p value < 0.05. Results: Findings revealed that 85.2% of the isolates carried Class 1 integrons, whereas 23.2% contained Class 2 integrons. Integron‐positive isolates presented an increased level of antibiotic resistance, particularly to trimethoprim–sulfamethoxazole (75% and 88.9%) and nalidixic acid (90.9% and 91.7%). Additionally, over 88% of the isolates had AcrAB–TolC efflux pump genes. MDR rates were 75% among intI1‐positive isolates and 83.3% among intI2‐positive isolates. Conclusion: In conclusion, integrons contribute to the spread of antibiotic resistance among UPEC isolates, whereas the AcrAB–TolC efflux pump contributes to the ability of bacteria to expel toxic substances. There is a need for effective infection management policies and proper application of antibiotics to limit the dissemination of resistance genes. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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