Incorporating Hands-on Flow Cytometry Into a Medical Laboratory Science Program Curriculum.

INTRODUCTION: Through grant funding, faculty provided hands-on flow cytometry experience to students who were enrolled in the Fall 2018 immunology and/or the Spring 2019 advanced hematology courses. This study assessed the effect of including hands-on flow cytometry exposure to students in a univers...

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Publicado en:Clinical Laboratory Science Vol. 38; no. 1; pp. 53 - 59
Autores principales: CHILDS, RACHEL, RUFFY, CRISTINA, KRAJ, BARBARA, HOGLUND, LESLIE
Formato: research tables/charts Journal Article
Publicado: American Society for Clinical Laboratory Science Winter2025
Acceso en línea:Ver este registro en EBSCOhost
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      vid: 38
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      pub: American Society for Clinical Laboratory Science
      place: Dundee, Michigan
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        atl: Incorporating Hands-on Flow Cytometry Into a Medical Laboratory Science Program Curriculum.
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        au:
          CHILDS, RACHEL
          RUFFY, CRISTINA
          KRAJ, BARBARA
          HOGLUND, LESLIE
        affil: Old Dominion University
      sug:
        subj:
          Medical Laboratory Science Education
          Program Evaluation
          Curriculum
          Students, Health Occupations
          Education, Medical Laboratory Technology
          Human
          Qualitative Studies
          Thematic Analysis
          Hematology Education
          Allergy and Immunology Education
          Clinical Competence
          Education, Competency-Based
          Leukemia
          Lymphoma
          Immunophenotyping
          Lymphocyte Subsets
          HIV Infections
          Statistical Significance
          Data Analysis Software
          Descriptive Statistics
          Funding Source
      ab: INTRODUCTION: Through grant funding, faculty provided hands-on flow cytometry experience to students who were enrolled in the Fall 2018 immunology and/or the Spring 2019 advanced hematology courses. This study assessed the effect of including hands-on flow cytometry exposure to students in a university-based medical laboratory science program. METHODS: Students spent approximately 4 hours processing, staining, and analyzing samples from a nearby hospital facility. In immunology, students were tasked with identifying lymphocyte subset populations in whom comprehension was assessed through short-essay and multiple-choice questions on an online homework quiz. In advanced hematology, students were tasked with immunophenotyping leukemia/lymphoma samples, in which comprehension was assessed via interpretation of patient scattergrams and correlation of results with the corresponding patient diagnoses. RESULTS: All students (100%) correctly interpreted a histogram presented to them in both the immunology and advanced hematology courses. A survey assessed students' perceptions of the effectiveness, strengths, and weaknesses of both exercises. Ninety-one percent and 100% of respondents agreed or strongly agreed that the hands-on exercise enhanced their understanding the flow cytometry principles underlying lymphocyte subset analysis and leukemia/lymphoma immunophenotyping, respectively. Respondents reported the experience as "extremely helpful" and that it allowed them to "correlate what was taught in class." DISCUSSION: Results indicated successful comprehension of basic lymphocyte and leukemia/lymphoma subset analysis. It was apparent that the activity helped students apply these principles toward their understanding of the material. Incorporating flow cytometry during didactic was beneficial, as it provided students with an experience they might not have gained during rotations.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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