Rule-Based Synthesis of Microscopy Images by Diffusion Refinement.

Abstract: Deep learning methods in medical imaging often suffer from the limited availability of high-quality annotated data, especially for rare conditions. This data scarcity is largely due to the need for domain expertise and the time-consuming process of data collection and annotation. Recent ad...

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Publicado en:Journal of Imaging Informatics in Medicine pp. 1 - 15
Autores principales: Kollár, Maroš, Vajsová, Andrea, Benesova, Wanda
Formato: Journal Article
Publicado: Springer Nature Apr2026
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Apr2026
      pid: 237
      pub: Springer Nature
      place: New York, New York
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        193274989
        10.1007/s10278-026-01935-x
        193274989
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        atl: Rule-Based Synthesis of Microscopy Images by Diffusion Refinement.
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          Kollár, Maroš
          Vajsová, Andrea
          Benesova, Wanda
        affil: Faculty of Informatics and Information Technologies, Slovak University of Technology
      sug:
      ab: Abstract: Deep learning methods in medical imaging often suffer from the limited availability of high-quality annotated data, especially for rare conditions. This data scarcity is largely due to the need for domain expertise and the time-consuming process of data collection and annotation. Recent advances in generative neural networks offer a promising solution by producing realistic synthetic images that can supplement or partially replace scarce real data. In this work, we propose a framework for synthesizing realistic microscopy images together with their corresponding structural annotations. The proposed method combines a procedural generator that encodes expert-defined diagnostic rules with a diffusion-based refinement that enhances visual realism while preserving the prescribed structure. We further introduce a multi-stage diffusion-based refinement process that utilizes a segmentation mask to guide refinement and ensure a predefined structure. We demonstrate the ability of the proposed framework on the use case of synthesizing microscopic images of motile cilia cross-sections, which are important for the diagnosis of Primary Ciliary Dyskinesia (PCD). Our results show that data created by the proposed approach can serve as both a complement to and a substitute for real training data in a downstream segmentation task.Graphical Abstract: Deep learning methods in medical imaging often suffer from the limited availability of high-quality annotated data, especially for rare conditions. This data scarcity is largely due to the need for domain expertise and the time-consuming process of data collection and annotation. Recent advances in generative neural networks offer a promising solution by producing realistic synthetic images that can supplement or partially replace scarce real data. In this work, we propose a framework for synthesizing realistic microscopy images together with their corresponding structural annotations. The proposed method combines a procedural generator that encodes expert-defined diagnostic rules with a diffusion-based refinement that enhances visual realism while preserving the prescribed structure. We further introduce a multi-stage diffusion-based refinement process that utilizes a segmentation mask to guide refinement and ensure a predefined structure. We demonstrate the ability of the proposed framework on the use case of synthesizing microscopic images of motile cilia cross-sections, which are important for the diagnosis of Primary Ciliary Dyskinesia (PCD). Our results show that data created by the proposed approach can serve as both a complement to and a substitute for real training data in a downstream segmentation task.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Unknown
    language: English
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